储存环境对鸡蛋壳表面3种沙门氏菌存活与迁移的影响

doi:10.16431/j.cnki.1671-7236.2016.11.037

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 3059-3065.doi: 10.16431/j.cnki.1671-7236.2016.11.037

储存环境对鸡蛋壳表面3种沙门氏菌存活与迁移的影响

沈学怀¹, 翟银建², 张丹俊¹, 潘孝成¹, 赵瑞宏¹, 戴银¹, 胡晓苗¹, 朱传民¹

1. 安徽省农业科学院畜牧兽医研究所, 合肥 230031;
2. 阜南县畜牧兽医局, 阜南 236300

收稿日期:2016-05-11 出版日期:2016-11-20 发布日期:2016-11-18
通讯作者: 张丹俊 E-mail:zhangdj694@sina.com
作者简介:沈学怀(1986-),男,安徽合肥人,硕士,实习研究员,研究方向:禽类传染病,E-mail:xuehuaishen1986@163.com
基金资助:
安徽省农业科学院院长青年创新基金项目（15B0414）；国家现代农业产业技术体系项目（CARS-41）；安徽省农科院科技创新团队项目（11C0404）；国家自然科学基金项目（31302044）

Effects of Storage Conditions on Survival and Migration of Three Kinds of Salmonella on Eggshell

SHEN Xue-huai¹, ZHAI Yin-jian², ZHANG Dan-jun¹, PAN Xiao-cheng¹, ZHAO Rui-hong¹, DAI Yin¹, HU Xiao-miao¹, ZHU Chuan-min¹

1. Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;
2. Funan County Animal Husbandry and Veterinary Bureau, Funan 236300, China

Received:2016-05-11 Online:2016-11-20 Published:2016-11-18

摘要/Abstract

摘要：

为探明不同储存环境对蛋壳表面沙门氏菌存活和向蛋内迁移的影响，在鸡蛋壳表面人工接种肠炎沙门氏菌、鼠伤寒沙门氏菌和鸡白痢沙门氏菌，试验环境设定温度4、25和37℃，环境相对湿度（RH）设定为50%和90%，在不同的储存环境下，各个时间点检测蛋壳表面沙门氏菌数量与蛋内容物中沙门氏菌污染情况。结果显示，4℃环境下可显著减少蛋壳表面3种沙门氏菌的数量；50% RH、25和37℃环境下蛋壳表面3种沙门氏菌的数量均逐渐下降；90% RH、25℃环境下蛋壳表面肠炎沙门氏菌、鼠伤寒沙门氏菌在个别时间点出现数量反弹，其总体数量呈现下降趋势，37℃环境下鸡白痢沙门氏菌的数量快速下降，而肠炎沙门氏菌和鼠伤寒沙门氏菌的数量先下降后上升，并保持在接种的数量级水平。鸡蛋内容物的检测结果显示，肠炎沙门氏菌和鼠伤寒沙门氏菌在高温、高湿环境下更易被检出，而鸡白痢沙门氏菌始终未被检出。综上所述，低温、低湿可减少蛋壳表面沙门氏菌存活，并阻止其菌体向蛋内迁移，高温、高湿环境促进蛋壳表面沙门氏菌的增殖，增加了其向蛋内迁移的几率，不同血清型沙门氏菌在鸡蛋壳表面的存活和向蛋内迁移的侵袭力存在一定差异。

关键词: 鸡蛋; 沙门氏菌; 存活; 迁移; 鸡蛋内容物

Abstract:

In order to study the effects of storage conditions on survival and migration of three kinds of Salmonella,S.enteritidis,S.typhimurium and S.pullorum were artificially contaminated on eggshell.Different environment temperatures（4,25 and 37℃）and relative humidity（50% and 90%）were seted,and the number of Salmonellas on the surface of eggshell and egg contents were counted and detected on various time points.The results showed that 4℃ obviously reduced the number of three kinds of Salmonellas.At 50% RH,the number of three kinds of Salmonellas were reduced on eggshell surface at 25 and 37℃ environment.At 90% RH,despite the individual time points,the number of S.enteritidis and S.typhimurium presented downward trend at 25℃.And at the 37℃,the number of S.pullorum rapidly reduced,and the number of S.enteritidis and S.typhimurium firstly decreased and then increased,and finally maintained at the level of inoculated magnitude.The results of egg content detection showed that S.enteritidis and S.typhimurium were more likely to be detected in high temperature and high humidity environment,while S.pullorum was not detected in all kinds of experimental environment.The results suggested that low temperature and low humidity reduced Salmonellas survival on eggshell and inhibited it migration into the egg,and high temperature and high humidity could promote Salmonellas proliferation,increase the risk of its penetration into egg.There were some differences in the survival on eggshell and invasion between the different serotypes Salmonellas.

Key words: egg; Salmonella; survival; migration; egg content

中图分类号:

S852.6

沈学怀, 翟银建, 张丹俊, 潘孝成, 赵瑞宏, 戴银, 胡晓苗, 朱传民. 储存环境对鸡蛋壳表面3种沙门氏菌存活与迁移的影响[J]. 《中国畜牧兽医》, 2016, 43(11): 3059-3065.

SHEN Xue-huai, ZHAI Yin-jian, ZHANG Dan-jun, PAN Xiao-cheng, ZHAO Rui-hong, DAI Yin, HU Xiao-miao, ZHU Chuan-min. Effects of Storage Conditions on Survival and Migration of Three Kinds of Salmonella on Eggshell[J]. , 2016, 43(11): 3059-3065.

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储存环境对鸡蛋壳表面3种沙门氏菌存活与迁移的影响

Effects of Storage Conditions on Survival and Migration of Three Kinds of Salmonella on Eggshell

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