《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 2989-2996.doi: 10.16431/j.cnki.1671-7236.2016.11.027

• 遗传繁育 • 上一篇    下一篇

沼泽型水牛微卫星DNA的遗传多样性分析

邓廷贤, 庞春英, 朱鹏, 陆杏蓉, 段安琴, 梁贤威   

  1. 中国农业科学院广西水牛研究所, 广西水牛遗传繁育重点实验室, 南宁 530001
  • 收稿日期:2016-04-18 出版日期:2016-11-20 发布日期:2016-11-18
  • 通讯作者: 梁贤威 E-mail:liangbri@126.com
  • 作者简介:邓廷贤(1983-),湖南郴州人,硕士,助理研究员,研究方向:分子遗传学,E-mail:dengtxbri@126.com
  • 基金资助:

    广西科技攻关国际交流与合作项目(桂科合14123001-5);广西水产畜牧兽医局科技项目(桂渔牧科201452032)

Genetic Diversity Analysis of Microsatellite DNA Markers in Swamp Buffalo

DENG Ting-xian, PANG Chun-ying, ZHU Peng, LU Xing-rong, DUAN An-qin, LIANG Xian-wei   

  1. Guangxi Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Science, Nanning 530001, China
  • Received:2016-04-18 Online:2016-11-20 Published:2016-11-18

摘要:

为探究中国沼泽型水牛品种或类群的遗传多样性,本研究采用了沼泽型水牛30个微卫星标记结合LabChip芯片检测法对德昌水牛、德宏水牛、温州水牛、贵州水牛、西林水牛、富钟水牛及两个引进品种摩拉水牛、尼里-拉菲水牛等40个样本进行了检测和遗传多样性分析。结果表明,在30个微卫星DNA标记上共发现332个等位基因,平均基因多样性和PIC值分别为0.7808和0.7554。聚类分析结果显示,德宏水牛和德昌水牛先聚为一类,随后与富钟水牛、贵州水牛、温州水牛、西林水牛聚为一类;摩拉水牛与尼里-拉菲水牛聚为一类。本研究证实了所选的30个沼泽型水牛微卫星DNA标记可作为有效的遗传标记用于水牛品种间的遗传多样性分析,同时也丰富了当前的水牛微卫星标记资源。

关键词: 水牛; 微卫星; 遗传多样性; 芯片检测

Abstract:

This study was aimed to investigate the genetic diversity of swamp buffalo breeds in China.The analysis of genetic diversity was performed in 40 buffalo individuals from 8 buffalo breeds(Dechang buffalo,Dehong buffalo,Wenzhou buffalo,Guizhou buffalo,Xilin buffalo,Fuzhong buffalo,Murrah buffalo and Nili-Ravi buffalo)by 30 microsatellite loci and LabChip chip test method.The results showed that 332 alleles at 30 microsatellite loci were found in 8 buffalo breeds,the average values of gene diversity and PIC were 0.7808 and 0.7554,respectively.Cluster analysis indicated that Dechang buffalo and Dehong buffalo firstly clustered together,followed by Fuzhong buffalo,Guizhou buffalo,Wenzhou buffalo and Xilin buffalo.Moreover,Murrah buffalo and Nili-Ravi buffalo clustered together.Our findings revealed that the 30 microsatellite loci could be used as an effective genetic marker for the analysis of genetic diversity among the buffalo breeds,which enriched the current SSR marker resources in buffalo.

Key words: buffalo; microsatellite; genetic diversity; chip test

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