家畜定时输精技术研究进展与应用现状

doi:10.16431/j.cnki.1671-7236.2016.07.026

摘要/Abstract

摘要：

随着人工授精技术在畜牧业中的广泛应用，优秀种公畜的利用率得到了极大提高。而人工授精成功的关键是输精的准确性和及时性，因此如何准确预测排卵时间及输精时间是提高人工授精效果的重要途径，也是当前畜牧工作者高度关注的问题。家畜的发情排卵受到体内生殖激素的精密调控，定时输精技术就是在利用生殖激素进行同期发情、同期排卵的基础上，通过调控母畜的发情和排卵来确定人工输精的最佳时间，以期达到提高家畜繁殖性能、减少发情鉴定工作量和促进养殖场批次化管理的目的。作者综述了家畜定时输精的原理及当前的应用现状，同时对其发展前景进行了展望，以期为畜牧业繁殖新技术的研究和应用提供参考依据。

关键词: 家畜; 人工授精; 定时输精; 生殖激素; 发情; 排卵

Abstract:

With the wide application of the artificial insemination (AI) in animal husbandry,the utilization rate of excellent sire got greatly improved.The key point of AI is predicting the time of ovulation and insemination accurately.It is also the point which the animal husbandry technologists highly concerned to improve the application effect of AI.Estrus and ovulation are regulated minutely by reproductive hormones in vivo in farm animals.Timed artificial insemination (TAI) is based on the synchronization of estrus and ovulation,both of which are triggered by reproductive hormones.So the optimal time for AI could be determined from the reproductive hormone treatment.The purpose of TAI is to improve the reproduction performance of farm animals,reduce the workload of identification of estrus,and promote batch management.This review mainly summerized the principles of TAI and its applications status,and the prospects was also discussed.It would provide a reference for the research and application of new reproductive technology in farm animals.

Key words: farm animals; AI; TAI; reproduction hormone; estrus; ovulation

中图分类号:

S814.3

吴俊辉, 曹丁壬, 刘艳婷, 高凤磊, 李莉, 吴同山, 张守全, 卫恒习. 家畜定时输精技术研究进展与应用现状[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(7): 1826-1833.

WU Jun-hui, CAO Ding-ren, LIU Yan-ting, GAO Feng-lei, LI Li, WU Tong-shan, ZHANG Shou-quan, WEI Heng-xi. Research Advances and Application Status of Timed Artificial Insemination (TAI) in Farm Animals[J]. , 2016, 43(7): 1826-1833.

参考文献

[1] 张莺莺.猪深部输精技术应用研究进展[J].中国畜牧兽医,2014,41(3):207-212.
[2] Menchaca A,Rubianes E.New treatments associated with timed artificial insemination in small ruminants[J].Reprod Fertil Dev,2004,16(4):403-413.
[3] 傅春泉,陈龙,季春雷.定时输精技术在规模奶牛场的应用[J].畜牧与兽医,2013,4:34-37.
[4] 王杏龙,戴荣亮,周俊波,等.母猪的同期发情配种试验[J].中国畜牧杂志,2006,13:18-19.
[5] Colazo M G,Mapletoft R J.A review of current timed-AI (TAI) programs for beef and dairy cattle[J].Can Vet J,2014,55(8):772-780.
[6] 陈志林,张常明,叶超,等.国外种猪繁殖技术进展[J].中国猪业,2015,3:37-41.
[7] Brüssow K P,Wähner M.Biological and technological background of estrus synchronization and fixed-time ovulation induction in the pig[J].Biotechnology in Animal Husbandry,2011,27(3):533-545.
[8] Holtz W,Sohnrey B,Gerland M,et al.Ovsynch synchronization and fixed-time insemination in goats[J]. Theriogenology,2008,69(7):785-792.
[9] 滕勇.GnRH、PGF_(2α)诱导奶牛同期发情效果的比较[J].黄牛杂志,2002,5:1-2.
[10] 曹晓云,王丽梅,史刚.不同方法诱导黄牛同期发情效果试验[J].云南畜牧兽医,2007,2:25-26.
[11] 姚国良.定时输精程序(Ovsynch/TAI)在奶牛繁殖上的应用效果[J].中国奶牛,2008,12:33-35.
[12] 高庆华,何良军,蒋涛,等.不同方法对受体母牛诱导同期发情效果的比较[J].畜牧与兽医,2006,1:62-63.
[13] 黄开华,陈江,张长号,等.同期排卵—定时输精技术在奶牛繁殖中的应用[J].黑龙江动物繁殖,2010,6:50-51.
[14] Pursley J R,Silcox R W,Wiltbank M C.Effect of time of artificial insemination on pregnancy rates,calving rates,pregnancy loss,and gender ratio after synchronization of ovulation in lactating dairy cows[J].J Dairy Sci,1998,81(8):2139-2144.
[15] Colazo M G,Gordon M B,Rajamahendran R,et al.Pregnancy rates to timed artificial insemination in dairy cows treated with gonadotropin-releasing hormone or porcine luteinizing hormone[J].Theriogenology,2009,72(2):262-270.
[16] Vasconcelos J L,Silcox R W,Rosa G J,et al.Synchronization rate,size of the ovulatory follicle,and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows[J].Theriogenology,1999,52(6):1067-1078.
[17] Souza A H,Ayres H,Ferreira R M,et al.A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows[J].Theriogenology,2008,70(2):208-215.
[18] Wiltbank M C,Souza A H,Carvalho P D,et al.Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle[J]. Reprod Fertil Dev,2011,24(1):238-243.
[19] Martinez M F,Kastelic J P,Adams G P,et al.The use of progestins in regimens for fixed-time artificial insemination in beef cattle[J].Theriogenology,2002,57(3):1049-1059.
[20] Moreira F,Orlandi C,Risco C A,et al.Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows[J].J Dairy Sci,2001,84(7):1646-1659.
[21] El-Zarkouny S Z,Cartmill J A,Hensley B A,et al.Pregnancy in dairy cows after synchronized ovulation regimens with or without presynchronization and progesterone[J].J Dairy Sci,2004,87(4):1024-1037.
[22] Galvāo K N,Sa F M,Santos J E.Reducing the interval from presynchronization to initiation of timed artificial insemination improves fertility in dairy cows[J].J Dairy Sci,2007,90(9):4212-4218.
[23] Dirandeh E,Roodbari A R,Colazo M G.Double-Ovsynch,compared with presynch with or without GnRH,improves fertility in heat-stressed lactating dairy cows[J].Theriogenology,2015,83(3):438-443.
[24] Lucy M C,Mcdougall S,Nation D P.The use of hormonal treatments to improve the reproductive performance of lactating dairy cows in feedlot or pasture-based management systems[J].Anim Reprod Sci,2004,82-83:495-512.
[25] Folman Y,Kaim M,Herz Z,et al.Comparison of methods for the synchronization of estrous cycles in dairy cows.2.Effects of progesterone and parity on conception[J].J Dairy Sci,1990,73(10):2817-2825.
[26] Xu Z Z,Burton L J,Macmillan K L.Reproductive performance of lactating dairy cows following estrus synchronization regimens with PGF2alpha and progesterone[J]. Theriogenology,1997,47(3):687-701.
[27] Small J A,Colazo M G,Kastelic J P,et al.Effects of progesterone presynchronization and eCG on pregnancy rates to GnRH-based,timed-AI in beef cattle[J].Theriogenology,2009,71(4):698-706.
[28] Mialot J P,Constant F,Dezaux P,et al.Estrus synchronization in beef cows:comparison between GnRH+PGF_2α+GnRH and PRID+PGF_2α+eCG[J].Theriogenology,2003,60(2):319-330.
[29] Ahuja C,Montiel F,Canseco R,et al.Pregnancy rate following GnRH + PGF_2α treatment of low body condition,anestrous Bos taurus by Bos indicus crossbred cows during the summer months in a tropical environment[J]. Animal Reproduction Science,2005,87(3-4):203-213.
[30] Sahu K,Parkinson T J,Laven R A.Conception rates to fixed-time artificial insemination of two oestrus synchronisation programmes in dairy heifers[J]. N Z Vet J, 2015,63(3):158-161.
[31] Swelum A,Alowaimer A N,Abouheif M A.Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes:Effects of hormonal profiles and reproductive performance[J]. Theriogenology,2015,84(4):498-503.
[32] Kusina N T,Tarwirei,Hamudikuwanda H,et al.A comparison of the effects of progesterone sponges and ear implants,PGF_2α,and their combination on efficacy of estrus synchronization and fertility of Mashona goat does[J].Theriogenology,2000,53(8):1567-1580.
[33] Martemucci G,D'Alessandro A G.Synchronization of oestrus and ovulation by short time combined FGA,PGF(2alpha),GnRH,eCG treatments for natural service or AI fixed-time[J].Anim Reprod Sci,2011,123(1-2):32-39.
[34] 王进荣,江国永,陈邦良,等.孕酮海绵栓制剂对提高波尔山羊繁殖率的研究[J].草食家畜,2000,4:21-23.
[35] 张一玲,渊锡藩,尹海科,等.山羊同期发情技术的应用[J].畜牧兽医杂志,2000,5:21-24.
[36] 杨梅,翁凡,汪立芹,等.利用CIDR+PMSG对乏情期绵羊同期发情处理的研究[J].草食家畜,2004,3:38-39.
[37] Brussow K P,Schneider F,Kanitz W,et al.Studies on fixed-time ovulation induction in the pig[J].Soc Reprod Fertil Suppl,2009,66:187-195.
[38] Cassar G,Kirkwood R N,Poljak Z,et al.Effect of single or double insemination on fertility of sows bred at an induced estrus and ovulation[J].J Swine Health Prod,2005,13(5):254-258.
[39] Knox R V,Taibl J N,Breen S M,et al.Effects of altering the dose and timing of triptorelin when given as an intravaginal gel for advancing and synchronizing ovulation in weaned sows[J].Theriogenology,2014,82(3):379-386.
[40] Stewart K R,Flowers W L,Rampacek G B,et al.Endocrine,ovulatory and reproductive characteristics of sows treated with an intravaginal GnRH agonist[J].Anim Reprod Sci,2010,120(1-4):112-119.
[41] Baer C,Bilkei G.The effect of intravaginal applied GnRH-agonist on the time of ovulation and subsequent reproductive performance of weaned multiparous sows[J].Reprod Domest Anim,2004,39(5):293-297.
[42] Driancourt M A,Cox P,Rubion S,et al.Induction of an LH surge and ovulation by buserelin (as receptal) allows breeding of weaned sows with a single fixed-time insemination[J].Theriogenology,2013,80(4):391-399.