›› 2015, Vol. 42 ›› Issue (12): 3288-3293.doi: 10.16431/j.cnki.1671-7236.2015.12.026

• 遗传繁育 • 上一篇    下一篇

猪血清中生长激素(GH)和生长激素释放激素(GHRH)的发育性变化研究

陶璇, 顾以韧, 梁艳, 杨雪梅, 曾凯, 陈晓晖, 杨跃奎, 钟志君, 吕学斌   

  1. 四川省畜牧科学研究院, 成都 610066
  • 收稿日期:2015-05-06 出版日期:2015-12-20 发布日期:2015-12-30
  • 通讯作者: 吕学斌 E-mail:lvxuebin@21cn.com
  • 作者简介:陶 璇(1982-),男,四川金堂人,硕士,助理研究员,研究方向:猪的遗传育种,E-mail:taoxuan0106@163.com 顾以韧(1980-),男,江苏盐城人,博士,副研究员,研究方向:动物遗传育种与繁殖,E-mail:guyiren1128@163.com
  • 基金资助:
    四川省基本科研业务费专项资金(SASA2014A04);国家科技支撑计划(2011BAD28B01-15);国家生猪产业技术体系-综合试验站(CARS-36);四川省"十二五"农作物及畜禽育种攻关(2011NZ0099-2);国家现代农业产业技术体系四川创新团队(sccxtd);四川省科技计划项目(2014NZ0092)

Study on Developmental Changes of Growth Hormone (GH) and Growth Hormone Releasing Hormone (GHRH) in Serum of Pigs

TAO Xuan, GU Yi-ren, LIANG Yan, YANG Xue-mei, ZENG Kai, CHEN Xiao-hui, YANG Yue-kui, ZHONG Zhi-jun, LV Xue-bin   

  1. Sichuan Academy of Animal Science, Chengdu 610066, China
  • Received:2015-05-06 Online:2015-12-20 Published:2015-12-30

摘要: 采用ELISA方法检测了3个不同体重梯度(90、110和130 kg)的川藏黑猪配套系和DLY猪血清中生长激素(GH)和生长激素释放激素(GHRH)的含量变化,并分析了其与肌纤维面积(CSA)和肌内脂肪(IMF)含量的关系。结果显示,90、110和130 kg体重梯度川藏黑猪配套系和DLY猪血清中GH含量分别为14.25、14.61、14.11及17.86、16.98、16.77 μg/L,GHRH含量分别为22.88、23.98、24.33及27.72、27.47、28.39 μg /L,除DLY猪90和130 kg血清中GH含量差异显著(P<0.05)外,品种内不同体重梯度间差异均不显著(P >0.05);不同猪种间,除110 kg梯度GHRH水平差异不显著(P >0.05)外,其余川藏黑猪配套系均显著低于DLY猪(P< 0.05)。相关性分析结果显示,GH、GHRH水平与CSA呈显著正相关(P< 0.05),与IMF含量呈极显著负相关(P< 0.01)。结果表明GH和GHRH可能通过血液对猪肌纤维的生长和肌内脂肪的沉积进行调控。

关键词: 川藏黑猪配套系; DLY; 肌纤维面积; 肌内脂肪; 生长激素; 生长激素释放激素

Abstract: The growth hormone (GH) and growth hormone releasing hormone (GHRH) contents in serum of 3 weights (90,110 and 130 kg) of Chuanzang Black swine hybrid and DLY pigs were detected by enzyme-linked immune sorbent assay (ELISA),their relationship with cross sectional area of muscle fiber (CSA) and intramuscular fat (IMF) content were also analyzed.The results showed that the GH contents of Chuanzang Black swine hybrid and DLY pigs of 3 weights were 14.25,14.61,14.11 and 17.86,16.98,16.77 μg/L,respectively,while the GHRH contents of which were 22.88,23.98,24.33 and 27.72,27.47,28.39 μg/L,respectively.There was no significant difference in different weight of the same breeds (P >0.05) except the significant difference between 90 and 130 kg of DLY pigs (P<0.05).The GH and GHRH contents of Chuanzang Black swine hybrid were both significant lower than DLY pigs of the same bodyweight (P< 0.05) except GHRH content of 110 kg weight (P >0.05).Relevance analysis showed that GH and GHRH contents had a significant positive correlation with CSA (P< 0.05),and an extremely significant negative correlation with IMF content (P< 0.01),which prompted that GH and GHRH might regulate the growth of muscle fiber and IMF deposition through blood.

Key words: Chuanzang Black swine hybrid; DLY; cross sectional area of muscle fiber (CSA); intramuscular fat (IMF); growth hormone (GH); growth hormone releasing hormone (GHRH)

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