蛋白质组学技术的应用研究

doi:10.16431/j.cnki.1671-7236.2015.11.017

摘要/Abstract

摘要： 蛋白质组学是探索细胞内蛋白质之间的相互作用及其变化规律的学科,近年来它被广泛应用于生命科学的各个领域,蛋白质组学还提供了一套在整体、动态水平上研究蛋白质的工具,大规模蛋白质组学技术的建立又极大地推动了蛋白质组学的研究进展。这些技术不但可以进行蛋白质的提取和分离,还可以进行目的蛋白的鉴定和分析。作者简述了蛋白质组学技术在疾病、皮肤、毛囊发育周期等方面的研究成果,以期为深入在绒毛用羊领域的研究提供借鉴。

关键词: 蛋白质组学; 绒毛用羊; 应用

Abstract: In the cell,proteomics is a discipline that explores the interactions and change rules between proteins.It is widely used in various fields of bioscience in recent years.Proteomics provides a set of tool on the whole and dynamic level.The establishment of large-scale proteomics technology greatly promote the research progress of proteomics.These techniques are not only used for protein extraction and separation,but also can undertake the identification and analysis of target protein.This paper briefly described the results about the research of proteomics technology in disease,skin,hair follicle growth cycle,etc,in order to provide some references for further research of wool-sheep and cashmere-goat.

Key words: proteomics; wool-sheep and cashmere-goat; application

中图分类号:

S826.9⁺1

韩文静, 张燕军, 王乐乐, 李晓燕, 苏蕊, 王瑞军, 李金泉. 蛋白质组学技术的应用研究[J]. , 2015, 42(11): 2928-2934.

HAN Wen-jing, ZHANG Yan-jun, WANG Le-le, LI Xiao-yan, SU Rui, WANG Rui-jun, LI Jin-quan. Application Research on Proteomics Technology[J]. , 2015, 42(11): 2928-2934.

参考文献

[1] 王绍美.烟草基因组学研究方法篇:5.烟草蛋白质组学研究方法和应用[J].中国烟草科学,2011(5):99-101.
[2] Manfredo Q.Proteomics and automation[J]. Electrophoresis,1999,20(4-5):664-677.
[3] Wilkins M R,Sanchez J C,Gooley A A,et al.Progress with proteome projects:Why all proteins expressed by a genome should be identified and how to do it[J].Biotechnology & Genetic Engineering Reviews,1996,13:19-50.
[4] Wasinger V C,Cordwell S J,Cerpa-Poljak A,et al.Progress with gene-product mapping of the Mollicutes:Mycoplas magenitalium[J].Electrophoresis,1995,16(7):1090-1094.
[5] 方福德,高友鹤.2014蛋白质组学专刊序言[J].生物工程学报,2014,7:1001-1003.
[6] 郭春燕,詹克慧.蛋白质组学技术研究进展及应用[J].云南农业大学学报(自然科学版),2010,4:583-591.
[7] 桑石磊,谭玉梅,刘永翔,等.蛋白质组学关键技术及应用[J].农技服务,2014,4:177-179.
[8] 万晶宏,贺福初.蛋白质组技术的研究进展[J].科学通报,1999,9:904-911.
[9] Gygi S P,Corthals G L,Zhang Y,et al.Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology[J].Proc Natl Acad Sci USA,2000,97(17):9390-9395.
[10] 潘运刚.蛋白质组学研究进展[J].赤峰学院学报(自然科学版),2005,6:48-49.
[11] 詹显全,陈主初.蛋白质组中蛋白质鉴定技术的研究近况[J].国外医学(分子生物学分册),2002,3:129-133.
[12] 孔汉金.羊口疮病毒感染宿主细胞的蛋白质组变化及功能分析[D].武汉:华中农业大学,2014.
[13] 赵有璋.羊生产学[M].北京:中国农业出版社,2002.
[14] 徐昌芬,陈永珍,王晓冬.组织胚胎学[M].南京:东南大学出版社,2005.
[15] 吕雪峰,郑文新.绒山羊皮肤毛囊的研究进展与前景[J].中国畜牧兽医,2010,37(11):25-28.
[16] 马馨,陈洋,姜怀志.哺乳动物毛囊结构特征及调控机制[J].中国草食动物科学,2013,33(1):53-56.
[17] 张璐,张燕军,苏蕊,等.microRNA对皮肤毛囊发育的调控机制[J],遗传,2014,36(7):655-660.
[18] Laura A,Elaine F.The hair cycle[J].Journal of Cell Science,2006,119:391-293.
[19] 陈小强,马章全.绒毛用羊生产关键技术[J].新农业,2012,6:18-19.
[20] 石晶.中国绒毛用羊养殖技术推广相关主体行为研究[D].北京:中国农业大学,2014.
[21] Hanash S.Disease proteomics[J]. Nature,2003,422(6928):226-232.
[22] Kageyama S,Isono T,Iwaki H,et al.Identification by proteomic analysis of calreticulin as a marker for bladder cancer and evaluation of the diagnostic accuracy of its detection in urine[J].Clinical Chemistry (Washington,DC),2004,50(5):857-866.
[23] Lei T,Zhao X H,Jin S,et al.Discovery of potential bladder cancer biomarkers by comparative urine proteomics and analysis[J].Clinical Genitourinary Cancer,2013,1(1):56-62.
[24] Xiao Y T,Dao Z C,Yao L W,et al.Comparative analysis of serum proteomes:Discovery of proteins associated with osteonecrosis of the femoral head[J].Translational Research,2006,148(3):114-119.
[25] David B,Tomás S,Miguel B,et al.Usefulness of haptoglobin and serum amyloid a proteins as biomarkers for atherothrombotic ischemic stroke diagnosis confirmation[J].Atherosclerosis,2008,205(2):561-567.
[26] 姜兰香,庞传超,巫毅,等.中国汉族肢端恶性黑素瘤的差异蛋白质组学研究[J].吉林大学学报(医学版),2012,5:866-869.
[27] Ye Y B,Huang A M,Huang C Z.Comparative mitochondrial proteomic analysis of hepatocellular carcinoma from patients[J].Proteomics,Clinical applications,2013,7(5-6):403-415.
[28] Taverna F,Negri A,Piccinini R,et al.Characterization of cell wall associated proteins of a Staphylococcus aureus isolated from bovine mastitis case by a proteomic approach[J].Veterinary Microbiology,2006,119(2-4):240-247.
[29] 刘永杰,张克山,尚佑军,等.Asia 1型FMDV感染BHK-21细胞差异蛋白质点2-DE分析[J].畜牧兽医学报,2011,42(1):145-149.
[30] Margaret G.Observations on the abosasal proteome during Teladorsagia circumcincta infection in sheep[D].Edinburgh:The University of Edinburgh,2010.
[31] Qin Q M,Pei J W,Ancona V,et al.RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1 alpha in supporting Brucella replication[J].PLoS Pathogens (Online),2008,4(7):e1000110.
[32] Mujer C V,Wagner M A,Eschenbrenner M,et al.Global analysis of Brucella melitensis proteomes[J].New York Academy of Sciences Annals,2002,969:97-101.
[33] Yang Y L,Yin J G,Guo D J,et al.Immunization of mice with recombinant S-adenosyl-L-homocysteine hydrolase protein confers protection against Brucella melitensis infection[J].FEMS Immunology and Medical Microbiology,2010,61(2):159-167.
[34] Huang C M,Foster K W,DeSilva T,et al.Comparative proteomic profiling of murine skin[J].Journal of Investigative Dermatology,2003,121(1):51-64.
[35] 李晖,刘广忠,孙伟民,等.病理性瘢痕与正常皮肤的蛋白质组学研究[J].第四军医大学学报,2009,30(24):2925-2927.
[36] 胡志成,朱家源,朱斌,等.病理性瘢痕与正常皮肤的蛋白质组学研究[J].中国病理生理杂志,2011,27(9):1802-1806.
[37] 高丽霞,刘志红,张燕军,等.绒山羊皮肤蛋白双向电泳条件的优化与常见问题分析[J].农业生物技术学报,2014,2:249-256.
[38] 松杰.内蒙古绒山羊不同月份皮肤蛋白质组分析[D].呼和浩特:内蒙古农业大学,2012.
[39] 杨建成,王熙,刘海英,等.蛋白质组学技术及其在绒山羊产业研究中的潜在应用[J].畜牧兽医科技信息,2013,11:7-8.
[40] 高丽霞,张燕军,张文广,等.内蒙古白绒山羊毛囊发育周期蛋白质表达谱分析[J].农业生物术学报,2014,6:727-735.
[41] 朱增勇.我国与澳大利亚畜产品贸易分析[J].中国畜牧杂志,2009,45(10):49-52.
[42] 苟明霞,杨旭红,孟广利.羊毛角蛋白的提取方法[J].毛纺科技,2011,3:18-21.
[43] 王江波,刘建勇,王洁,等.羊毛角蛋白的提取[J].毛纺科技,2012,4:44-47.
[44] 王丽静,史吉华,周奥佳,等.新型羊毛角蛋白提取技术探究[J].毛纺科技,2013,5:1-6.
[45] Plowman J E,Jordan T W,Bryson W G.Application of proteomics for determining protein markers for wool quality traits[J].Electrophoresis,2000,21(9):1899-1906.
[46] 杨洁,付雪峰,黄锡霞,等.细毛羊皮肤组织中毛囊蛋白质2-DE图谱的建立与初步分析[J].中国畜牧兽医,2015,42(1):124-130.
[47] 张娟,陈青文,龚.基于蛋白质组学的羊绒鉴定法[A].中国化学会首届全国质谱分析学术研讨会会议论文集[C].中国化学会、国家自然科学基金委员会.2014.
[48] Youngmi K,Taewoo K,Hyung-Min C.Qualitative identification of cashmere and yak fibers by protein fingerprint analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J].Ind Eng Chem Res,2013,52(16):5563-5571.
[49] Caufield J H,Sakhawalkar N,Uetz P.A comparison and optimization of yeast two-hybrid systems[J]. Methods,2012,58(4):312-324.
[50] 杨娇馥.应用酵母双杂交技术鉴定绒山羊FAK相互作用蛋白TSC2和AKT[D].呼和浩特:内蒙古大学,2011.
[51] 王晓晶.绒山羊细胞中Rheb-FKBP38相互作用关系的蛋白质组学研究[D].呼和浩特:内蒙古大学,2012.