冷刺激小鼠脂肪组织差异表达长链非编码RNA的初步研究

doi:10.16431/j.cnki.1671-7236.2015.11.008

›› 2015, Vol. 42 ›› Issue (11): 2867-2872.doi: 10.16431/j.cnki.1671-7236.2015.11.008

冷刺激小鼠脂肪组织差异表达长链非编码RNA的初步研究

黄素娟¹, 陶聪¹, 甄二东¹, 王亚君¹, 魏刚², 白立景¹, 杨述林¹, 王彦芳¹, 李奎¹, 敖红¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 中国科学院动物研究所, 北京 100101

收稿日期:2015-03-30 出版日期:2015-11-20 发布日期:2015-11-26
通讯作者: 敖红 E-mail:aohong@caas.cn
作者简介:黄素娟(1988-),女,山东济宁人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:20083076@163.com
基金资助:
转基因新品种培育重大专项(2014ZX08011-006);中国农业科学院科技创新工程(ASTIP-IAS05)

Preliminary Study on Differentially Expressed Long Non-coding RNA in Cold-exposure Adipose Tissue of Mice

HUANG Su-juan¹, TAO Cong¹, ZHEN Er-dong¹, WANG Ya-jun¹, WEI Gang², BAI Li-jing¹, YANG Shu-lin¹, WANG Yan-fang¹, LI Kui¹, AO Hong¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Received:2015-03-30 Online:2015-11-20 Published:2015-11-26

摘要/Abstract

摘要： 脂肪组织主要有白色脂肪组织(WAT)和褐色脂肪组织(BAT)两种类型;WAT储存能量,BAT通过特异性表达解耦联蛋白1(uncoupling protein 1,UCP1)消耗能量产热。与BAT不同,WAT具有很强的可塑性,在寒冷刺激下,呈现褐色脂肪表型、获得褐色脂肪的产热活性("褐色化")。根据对冷刺激组(6℃)和对照组(28℃)小鼠的肩胛区褐色脂肪组织(iBAT)和皮下白色脂肪组织(sWAT)的RNA转录组测序分析结果,初步筛选出1个差异明显的长链非编码RNA (lncRNA-6030408B16Rik)。通过qRT-PCR检测lncRNA-6030408B16Rik在野生型小鼠的脾脏、肌肉、肾脏、十二指肠、大脑、肝脏、sWAT和iBAT中的表达;24只雄性C57BL/6野生型小鼠随机分成冷刺激组(6℃)和对照组(28℃),两组小鼠分别在6和28℃环境下处理10 d,分别采集两组的iBAT和sWAT;并分离出生1 d的野生型小鼠褐色前脂肪细胞,诱导分化后,收集0、1、3、5 d的细胞,检测UCP1基因和lncRNA-6030408B16Rik在分化过程中的时空表达。qRT-PCR结果显示iBAT和sWAT中lncRNA-6030408B16Rik的表达量均明显高于其他组织;与对照组相比,冷刺激组iBAT中lncRNA-6030408B16Rik的表达量极显著上调(P<0.01),冷刺激组sWAT中lncRNA-6030408B16Rik的表达量显著上调(P<0.05);前脂肪细胞诱导分化的过程中,lncRNA-6030408B16Rik的表达量呈先上调后下调的趋势。lncRNA-6030408B16Rik在脂肪组织中特异性高表达。lncRNA-6030408B16Rik可能对白色脂肪组织"褐色化"及褐色前脂肪细胞的分化具有重要的调控作用。

关键词: 小鼠; 白色脂肪组织; 褐色脂肪组织; 前脂肪细胞分化; 长链非编码RNA

Abstract: Adipose tissue had two different types:White adipose tissue (WAT) that stored energy and brown adipose tissue (BAT) which could consume energy by specific expression of uncoupling protein 1(UCP1).In contrast with BAT,WAT had strong plasticity and possessed capacity of conversing phenotype,which was called thermogenesis (browning) under cold exposure.According to the results of RNA transcriptome sequencing of interscapular brown adipose tissue (iBAT) and subcutaneous white adipose tissue (sWAT) in the cold exposure group and control group,we preliminary screened one differentially expressed lncRNA (lncRNA-6030408B16Rik).The lncRNA-6030408B16Rik expression pattern was detected by qRT-PCR in spleen,muscle,kidney,duodenum,brain,liver,sWAT and iBAT from the wild type mice.Twenty-four 8-week-old C57BL/6 wild type mice were randomly divided into two groups respectively under cold exposure 6℃ and 28℃ as control,followed by qRT-PCR detection of lncRNA-6030408B16Rik expression in sWAT and iBAT.Separating preadipocytes from 1-day-old wild type mice,then inducing differentiation,and collecting cells on 0,1,3 and 5 days,expression of UCP1 and lncRNA-6030408B16Rik were detected by qRT-PCR.The results showed that the expression of lncRNA-6030408B16Rik were greatly enriched both in sWAT and iBAT compared with other six tissues.The expression of lncRNA-6030408B16Rik were significantly or extremely significantly up-regulated both in sWAT and iBAT of mice under cold exposure compared to control group (P<0.05;P<0.01).In contrast with UCP1,lncRNA-6030408B16Rik expression level presented an up-down trend at 0,1,3 and 5 days of brown preadipocytes differentiation.lncRNA-6030408B16Rik showed tissue-specific expression both in sWAT and iBAT,these results suggested that it might play important roles in WAT "browning" and brown preadipocytes differentiation.

Key words: mice; white adipose tissue; brown adipose tissue; preadipocytes differentiation; long non-coding RNA (lncRNA)

中图分类号:

黄素娟, 陶聪, 甄二东, 王亚君, 魏刚, 白立景, 杨述林, 王彦芳, 李奎, 敖红. 冷刺激小鼠脂肪组织差异表达长链非编码RNA的初步研究[J]. , 2015, 42(11): 2867-2872.

HUANG Su-juan, TAO Cong, ZHEN Er-dong, WANG Ya-jun, WEI Gang, BAI Li-jing, YANG Shu-lin, WANG Yan-fang, LI Kui, AO Hong. Preliminary Study on Differentially Expressed Long Non-coding RNA in Cold-exposure Adipose Tissue of Mice[J]. , 2015, 42(11): 2867-2872.

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冷刺激小鼠脂肪组织差异表达长链非编码RNA的初步研究

Preliminary Study on Differentially Expressed Long Non-coding RNA in Cold-exposure Adipose Tissue of Mice

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