转基因猪中抗生素标记基因neo漂移风险评估

doi:10.16431/j.cnki.1671-7236.2015.10.031

›› 2015, Vol. 42 ›› Issue (10): 2720-2725.doi: 10.16431/j.cnki.1671-7236.2015.10.031

转基因猪中抗生素标记基因neo漂移风险评估

王庆庆¹, 高鹏飞¹, 李和刚², 马德尊¹, 蔡春波¹, 钱丽丽¹, 姜声旺¹, 崔文涛¹

1. 中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与种质创新重点实验室, 北京 100193;
2. 青岛畜牧兽医研究所, 青岛 266100

收稿日期:2015-03-16 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 崔文涛 E-mail:cuiwentao@caas.cn
作者简介:王庆庆(1989-),男,山东临沂人,硕士,研究方向:转基因动物安全评价,E-mail:nkywqq@163.com
基金资助:
主要转基因动物和饲料安全性检测及安全控制关键技术引进(2011-G11);猪经济性状的功能基因组学研究(2013AA102502);中国农业科学院科技创新工程(ASTIP-IAS05)

Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs

WANG Qing-qing¹, GAO Peng-fei¹, LI He-gang², MA De-zun¹, CAI Chun-bo¹, QIAN Li-li¹, JIANG Sheng-wang¹, CUI Wen-tao¹

1. Key Laboratory of Farm Animal Genetic Resources and Germplam Innovation, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Qingdao Institute of Animal Science and Veterinary Medicine, Qingdao 266100, China

Received:2015-03-16 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 本试验旨在研究转基因猪中抗生素标记基因neo漂移的可能性。利用Southern blotting鉴定F1代仔猪的显隐性,结果发现6头仔猪中有3头为转基因仔猪,3头为阴性仔猪。通过PCR技术对试验仔猪血液和消化道组织中neo基因进行检测,结果发现neo基因没有在血液水平和消化道组织中发生漂移。通过PCR技术对试验仔猪肠道细菌中neo基因进行检测,在肠道细菌基因组中没有检测到neo基因的存在。检测结果表明,仔猪血液、肠道细菌和消化道组织等都没有发生neo基因的漂移。

关键词: 转基因仔猪; neo基因; 基因漂移

Abstract: This study was designed to study the possibility of gene drift of the antibiotic marker gene neo in transgenic pigs.First,dominance and recessiveness of neo gene in 6 piglets of F1 were identified using Southern blotting.It was found that 3 piglets were positive transgenic piglets and the other 3 piglets were non-transgenic piglets.The neo gene in blood and digestive tract tissue of experimental piglets were detected using PCR.The result showed that no gene drift was showed in blood and digestive tract tissues of experimental piglets.Then PCR technology was used to detect neo gene in intestinal bacteria of experimental piglets.The result showed that neo gene did not exist in the genome of intestinal bacteria.This study suggested that no gene drift was found in blood,intestinal bacteria and digestive tract tissues of piglets.

Key words: transgenic piglets; neo gene; gene drift

中图分类号:

王庆庆, 高鹏飞, 李和刚, 马德尊, 蔡春波, 钱丽丽, 姜声旺, 崔文涛. 转基因猪中抗生素标记基因neo漂移风险评估[J]. , 2015, 42(10): 2720-2725.

WANG Qing-qing, GAO Peng-fei, LI He-gang, MA De-zun, CAI Chun-bo, QIAN Li-li, JIANG Sheng-wang, CUI Wen-tao. Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs[J]. , 2015, 42(10): 2720-2725.

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转基因猪中抗生素标记基因neo漂移风险评估

Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs

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