载脂蛋白CⅡ基因在猪肝脏组织中的发育性表达研究

doi:10.16431/j.cnki.1671-7236.2015.10.032

›› 2015, Vol. 42 ›› Issue (10): 2726-2731.doi: 10.16431/j.cnki.1671-7236.2015.10.032

载脂蛋白CⅡ基因在猪肝脏组织中的发育性表达研究

刘翠翠^1,2, 郭晓红¹, 曹果清¹, 高鹏飞¹, 浦忠得¹, 牛姣艳¹, 张旗¹, 李萌¹, 靳玉舒¹, 李步高¹

1. 山西农业大学动物科技学院, 太谷 030801;
2. 临县高级中学, 临县 033299

收稿日期:2015-03-27 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 李步高 E-mail:jinrenn@163.com
作者简介:刘翠翠(1989-),女,山西石楼人,硕士生,研究方向:猪的分子育种,E-mail:870053703@qq.com
基金资助:
山西省科技创新重点团队项目(2013131019);山西省农业科技攻关项目(20130311026-1、20140311020-5);山西省科技成果推广项目(2012072008)

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

LIU Cui-cui^1,2, GUO Xiao-hong¹, CAO Guo-qing¹, GAO Peng-fei¹, PU Zhong-de¹, NIU Jiao-yan¹, ZHANG Qi¹, LI Meng¹, JIN Yu-shu¹, LI Bu-gao¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Middle School of Lin Country, Linxian 033299, China

Received:2015-03-27 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 试验旨在探究载脂蛋白CⅡ(apolipoprotein Ⅱ,ApoCⅡ)基因mRNA在马身猪和大白猪肝脏组织中的发育性表达规律,揭示ApoCⅡ基因表达水平与猪脂质代谢之间的关系。采用实时荧光定量PCR技术检测了马身猪和大白猪1~180日龄(1、30、60、90、120、150和180日龄)肝脏组织中ApoCⅡ基因mRNA表达。结果表明,马身猪和大白猪肝脏组织ApoCⅡ基因mRNA发育性变化趋势存在差异,马身猪ApoCⅡ表达量从1日龄到60日龄逐渐降低,在90日龄时上升,之后逐渐降低;而大白猪从1日龄到150日龄呈现逐渐降低趋势,180日龄时回升。1日龄时,马身猪和大白猪肝脏中ApoCⅡ基因mRNA的表达量差异不显著(P>0.05),在其他阶段,两品种间的表达量存在显著或极显著差异(P<0.05或 P<0.01)。这表明肝脏中ApoCⅡ mRNA的表达具有显著的日龄依赖性和明显的品种差异性,其表达可能对猪脂质代谢有显著影响。

关键词: 猪; ApoCⅡ基因; 发育性表达; 肝脏

Abstract: The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.

Key words: pig; ApoCⅡ gene; developmental expression; liver

中图分类号:

S813.3

刘翠翠, 郭晓红, 曹果清, 高鹏飞, 浦忠得, 牛姣艳, 张旗, 李萌, 靳玉舒, 李步高. 载脂蛋白CⅡ基因在猪肝脏组织中的发育性表达研究[J]. , 2015, 42(10): 2726-2731.

LIU Cui-cui, GUO Xiao-hong, CAO Guo-qing, GAO Peng-fei, PU Zhong-de, NIU Jiao-yan, ZHANG Qi, LI Meng, JIN Yu-shu, LI Bu-gao. Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs[J]. , 2015, 42(10): 2726-2731.

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载脂蛋白CⅡ基因在猪肝脏组织中的发育性表达研究

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

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