GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究

doi:10.16431/j.cnki.1671-7236.2015.07.028

›› 2015, Vol. 42 ›› Issue (7): 1816-1822.doi: 10.16431/j.cnki.1671-7236.2015.07.028

GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究

路佩瑶¹, 王福传¹, 宋献艺¹, 张凯¹, 张宁¹, 岳文斌²

1. 山西省农业科学院饲料兽药研究所, 太原 030031;
2. 山西农业大学动物科技学院, 太谷 030801

修回日期:2015-04-20 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 岳文斌 E-mail:ywbsxau@126.com
作者简介:路佩瑶(1984-),女,山西左权人,博士,助理研究员,研究方向:动物繁殖调控技术,E-mail:peiyao_lu@163.com
基金资助:
山西省农业科学院博士基金项目(YBSJJ1403);现代农业产业技术体系建设专项资金(NYCYTX-39)

Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep

LU Pei-yao¹, WANG Fu-chuan¹, SONG Xian-yi¹, ZHANG Kai¹, ZHANG Ning¹, YUE Wen-bin²

1. Feed and Veterinary Drug Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
2. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

Revised:2015-04-20 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 试验旨在研究GPR54(G-protein coupled receptor 54)基因在绵羊不同组织中的表达及其在4~6月龄公羔睾丸组织中的表达特性及定位。本研究采用实时荧光定量PCR法检测各组织GPR54 mRNA表达规律,以及其在不同月龄公羔睾丸中的表达量,运用免疫组化技术对睾丸中GPR54基因进行定位分析。结果显示,GPR54在不同组织中均有不同程度的表达,其中在下丘脑、垂体和睾丸中大量表达;GPR54在4~6月龄绵羊公羔睾丸组织中呈阶段性表达,6月龄表达量显著高于4和5月龄(P<0.05);4月龄时,在精原细胞和极少数的初级精母细胞中检测到较弱的阳性信号;6月龄性成熟时,在睾丸中分裂早期的精子细胞检测到强阳性信号。综上表明,GPR54基因在绵羊不同组织中广泛表达,在下丘脑-垂体-睾丸生殖轴中发挥重要的作用,并与动物的性成熟及雄性动物精子发生过程有密切的关系。

关键词: GPR54基因; 绵羊; 实时荧光定量PCR; 免疫组化; 睾丸

Abstract: The objectives of this study were to evaluate the expression of G-protein coupled receptor 54 (GPR54) in various tissues and the cellular localization of testis of 4 to 6-month-old sheep.In this experiment,mRNA expression was detected by Real-time fluorescence quantitative PCR.Cellular localization of GPR54 in testis was examined by immunohistochemistry.The results showed that GPR54 mRNA was expressed in all the tissues,and abundantly expressed in hypothalamus,pituitary gland and testis.The expression level gradually increased with the individual growth and development,the mRNA and protein expression of GPR54 in testis of 6-month-old was significantly higher than 4-month-old and 5-month-old (P<0.05);It was detected that GPR54 expression only in spermatogonia and a very number of primary spermatocytes in the testis by immunohistochemical staining,and it could detect significantly that the GPR54 expression in many split early sperm cells.The results demonstrated that it had close relationship between GPR54 and sexually mature of animals and the process of spermatogenesis in male animals.

Key words: GPR54 gene; sheep; Real-time fluorescence quantitative PCR; immunohistochemical; testis

中图分类号:

S814.8

路佩瑶, 王福传, 宋献艺, 张凯, 张宁, 岳文斌. GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究[J]. , 2015, 42(7): 1816-1822.

LU Pei-yao, WANG Fu-chuan, SONG Xian-yi, ZHANG Kai, ZHANG Ning, YUE Wen-bin. Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep[J]. , 2015, 42(7): 1816-1822.

参考文献

[1] Rhie Y J.Kisspeptin/G protein-coupled receptor-54 system as an essential gatekeeper of pubertal development[J].Ann Pediatr Endocrinol Metab, 2013,18(2):55-59.
[2] Stafford L J,Xia C,Ma W,et al.Identification and characterization of mouse metastasis-suppressor Kiss1 and its G-protein-coupled receptor[J].Cancer Res, 2002,62(19):5399-5404.
[3] Ohtaki T,Shintani Y,Honda S,et al.Metastasis suppressor gene Kiss-1 encodes peptide ligand of a G-protein coupled receptor[J].Nature, 2001,411(6837):613-617.
[4] Dhaur D K,Naora H,Kubota H,et al.Downregulation of Kiss-1 expression is responsible for tumor invasion and worse prognosis in gastric carcinoma[J].Int J Cancer, 2004,111(6):868-872.
[5] Smith J T,Clarke U.Kisspeptin expression in the brain:Catalyst for the initiation of puberty[J].Rev Endor Metab Disord,2007,8(1):1-9.
[6] Yeo S H,Clarkson J,Herbison A E,et al.Kisspeptin-GPR54 signaling at the GnRH neuron is necessary for negative feedback regulation of luteinizing hormone secretion in female mice[J].Neuroendocrinology,2014,100(2-3):191-197.
[7] Funes S,Hedrick J A,Vassileva G,et al.The Kiss-1 receptor GPR54 is essential for the development of the murine reproductice system[J].Biochemical Biophys Res Commun,2003,312(4):1357-1363.
[8] Franceschini I,Lomet D,Cateau M,et al.Kisspeptin immunoreactive cells of the ovine preoptic area and arcuate nucleus co-express estrogen receptor alpha[J].Neurosci Lett,2006,40(3):225-230.
[9] Messager S,Chatzidaki E F,Ma D,et al.Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-couple receptor 54[J].Proc Natl Acad Sci USA,2005,102(5):1761-1766.
[10] Gottsch M L,Cunningham M J,Smith J T,et al.A role for kisspeptins in the regulation of gonadotropin secretion in the mouse[J].Endocrinoloty, 2004,145(9):4073-4077.
[11] 荀文娟,周汉林,侯冠彧,等.亲吻素-1基因对雄性哺乳动物生殖调控的研究进展[J].中国畜牧兽医,2014,41(1):171-174.
[12] Clarke I J,Smith J T,Caraty A,et al.Kisspeptin and seasonality in sheep[J].Peptides,2009,30(1):154-163.
[13] 王雪松.Kiss-1/GPR54系统在雌性大鼠生殖轴系功能研究[D].南京:南京医科大学,2007.
[14] Pasquier J,Kamech N,Lafont A G,et al.Molecular evolution of GPCRs:Kisspeptin/kisspeptin receptors[J].J Mol Endocrinol, 2014,52(3):101-117.
[15] Aragno M,Cutrin J C,Mstrocola R,et al.Oxidative stress and kidney dysfunction due to ischemia/reperfusion in rat:Attenuation by dehydroepiandrosterone[J].Kidney International, 2003,64(3):836-843.
[16] Selzner N,Rudiger H,Graf R,et al.Protective strategies against ischemic injury of the liver[J].Gastroenterology, 2003,125(3):917-936.

GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究

Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep

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