miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响

doi:10.16431/j.cnki.1671-7236.2015.07.009

›› 2015, Vol. 42 ›› Issue (7): 1680-1685.doi: 10.16431/j.cnki.1671-7236.2015.07.009

miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响

谢雪娇, 栾生, 王媛, 张莉, 崔英俊, 王春梅, 李庆章

东北农业大学乳品科学教育部重点实验室, 哈尔滨 150030

收稿日期:2014-12-23 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 王春梅 E-mail:wangcm-1@126.com
作者简介:谢雪娇(1988-),女,吉林昌邑人,硕士,研究方向:微小RNA在乳腺发育泌乳中的作用,E-mail:jiaotao819@163.com
基金资助:
黑龙江省教育厅重点项目(1154z1004)

Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein

XIE Xue-jiao, LUAN Sheng, WANG Yuan, ZHANG Li, CUI Ying-jun, WANG Chun-mei, LI Qing-zhang

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China

Received:2014-12-23 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： MicroRNAs(miRNAs)是一类长度约为22 nt的非编码的调控性小RNA,在诸多生命活动中发挥重要作用,如参与调控细胞的增殖、分化、凋亡及肿瘤的发生发展。本试验应用脂质体转染技术抑制miR-142-3p在人乳腺上皮细胞的表达。试验采用实时荧光定量PCR、Western blotting、细胞增殖分析等技术,探索miR-142-3p对人乳腺上皮细胞增殖及乳蛋白合成的影响。结果显示,miR-142-3p沉默后,催乳素受体(prolactin receptor,PRLR)蛋白表达增强,同时,相关通路蛋白AKT、mTOR、STAT5、cyclinD1表达量均增加,细胞增殖能力增强。结果表明,在人乳腺上皮细胞中,miR-142-3p的沉默使PRLR蛋白表达量升高,通过调控AKT、mTOR、STAT5、cyclinD1相关通路蛋白而促进乳蛋白质的合成和乳腺上皮细胞的增殖。

关键词: miR-142-3p; 催乳素受体; 乳腺发育; 增殖

Abstract: MicroRNAs (miRNAs) are a group of small,non-coding RNA molecules about 22 nucleotides to regulate a wide variety of important biological processes,including cell proliferation,differentiation,apoptosis as well as the progression of tumors.Liposome transfection was used to detect the expression of miR-142-3p in human mammary epithelial cells,the effects of miR-142-3p on the cell proliferation,apoptosis and milk protein synthesis were detected by Real-time PCR,Western blotting,cell proliferation analysis.The results indicated that after miR-142-3p being silenced,prolactin receptor (PRLR) protein was increased,at the same time the expressions of related pathways protein AKT,mTOR,STAT5 and cyclinD1 were increased,the ability of cell proliferation was increased.The results suggested that in human mammary epithelial cells,the silence of miR-142-3p could increase the expression of PRLR protein,miR-142-3p could promote the synthesis of milk protein and increase the proliferation of mammary epithelial cells by regulating related pathways proteins AKT,mTOR,STAT5 and cyclinD1.

Key words: miR-142-3p; prolactin receptor (PRLR); mammary gland development; proliferation

中图分类号:

Q527

谢雪娇, 栾生, 王媛, 张莉, 崔英俊, 王春梅, 李庆章. miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响[J]. , 2015, 42(7): 1680-1685.

XIE Xue-jiao, LUAN Sheng, WANG Yuan, ZHANG Li, CUI Ying-jun, WANG Chun-mei, LI Qing-zhang. Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein[J]. , 2015, 42(7): 1680-1685.

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miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响

Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein

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