五指山猪线粒体控制区序列分析及遗传多样性研究

doi:10.16431/j.cnki.1671-7236.2015.05.029

›› 2015, Vol. 42 ›› Issue (5): 1222-1231.doi: 10.16431/j.cnki.1671-7236.2015.05.029

五指山猪线粒体控制区序列分析及遗传多样性研究

于萍^1,2, 曹婷², 施力光², 刘雅芳¹, 赵春萍^1,2, 张立岭¹, 侯冠彧²

1. 海南大学农学院, 海口 570228;
2. 中国热带农业科学院热带作物品种资源研究所, 儋州 571737

收稿日期:2014-10-10 出版日期:2015-05-20 发布日期:2015-06-02
通讯作者: 侯冠彧 E-mail:guanyuhou@126.com
作者简介:于萍(1988-),女,山东烟台人,硕士生,研究方向:家畜遗传资源,E-mail:yuping198807@126.com
基金资助:
家养动物种质资源平台运行服务;中央级公益性科研院所基本业务费(1630032013020)

Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig

YU Ping^1,2, CAO Ting², SHI Li-guang², LIU Ya-fang¹, ZHAO Chun-ping^1,2, ZHANG Li-ling¹, HOU Guan-yu²

1. College of Agriculture, Hainan University, Haikou 570228, China;
2. Tropical Crops Genetic Resource Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received:2014-10-10 Online:2015-05-20 Published:2015-06-02

摘要/Abstract

摘要： 为了研究五指山猪遗传多样性和系统地位,本研究采用PCR产物直接测序法得到55条五指山猪线粒体控制区(mtDNA D-Loop)全序列,并结合GenBank中发表的1条五指山猪mtDNA D-Loop全序列进行结构分析、遗传多样性研究及系统发育分析。结果发现,56条序列中出现21次保守区变异,即"TTATAAAACAC"序列重复,共定义13个单倍型,发现14个变异位点,单倍型多样度(Hd)和核苷酸多样度(Pi)分别为0.838和0.00334,表明五指山猪遗传多样性较丰富。五指山猪线粒体控制区序列构建的系统发育树和Network网络分析呈现两大分支,推测有两个母系起源,且与GenBank中其他23个猪种的聚类结果表明,五指山猪与长江流域以南地区的猪种有着较近的亲缘关系。

关键词: 五指山猪; 线粒体控制区; 遗传多样性

Abstract: In order to research the genetic diversity and phylogenies of Wuzhishan pig, 56 complete mtDNA D-Loop were analyzed.55 sequences were amplified by polymerase chain reaction (PCR) and 1 sequence was downloaded from GenBank.The results showed that repetitive sequence "TTATAAAACAC" appeared in the conservative regions of 21 mtDNA D-Loop sequences.13 haplotypes and 14 variable sites were observed in 56 sequences.Haplotype diversity and nucleotide diversity (Hd =0.838 and Pi=0.00334) indicated that Wuzhishan pig had high genetic diversity.Two branches in the phylogenetic tree and Network analysis diagram indicated that there were two maternal origins in Wuzhishan pig.The phylogenetic tree of Wuzhishan pig and other 23 species showed that Wuzhishan pig had close genetic relationship with the pig breeds in the south region of the Yangtze river.

Key words: Wuzhishan pig; mitochondrial DNA D-Loop; genetic diversity

中图分类号:

S813.3

于萍, 曹婷, 施力光, 刘雅芳, 赵春萍, 张立岭, 侯冠彧. 五指山猪线粒体控制区序列分析及遗传多样性研究[J]. , 2015, 42(5): 1222-1231.

YU Ping, CAO Ting, SHI Li-guang, LIU Ya-fang, ZHAO Chun-ping, ZHANG Li-ling, HOU Guan-yu. Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig[J]. , 2015, 42(5): 1222-1231.

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五指山猪线粒体控制区序列分析及遗传多样性研究

Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig

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