口蹄疫病毒前导蛋白酶抑制宿主先天性免疫应答的机制

doi:10.16431/j.cnki.1671-7236.2015.04.039

›› 2015, Vol. 42 ›› Issue (4): 1028-1031.doi: 10.16431/j.cnki.1671-7236.2015.04.039

口蹄疫病毒前导蛋白酶抑制宿主先天性免疫应答的机制

李夏莹¹, 许文涛^2,3, 汪其怀¹

1. 农业部科技发展中心, 北京 100122;
2. 中国农业大学食品科学与营养工程学院, 北京 100083;
3. 农业部转基因生物食用安全监督检验测试中心(北京), 北京 100083

收稿日期:2014-10-31 出版日期:2015-04-20 发布日期:2015-05-05
通讯作者: 汪其怀 E-mail:esmacloed006@163.com
作者简介:李夏莹(1986-),女,云南玉溪人,博士,从事口蹄疫病毒蛋白与宿主细胞相互作用机制方面的研究,E-mail:esmacloed006@163.com
基金资助:
转基因生物新品种培育重大专项(2014ZX0801103B);食品质量与安全北京实验室

Mechanism of Foot-and-mouth Disease Virus Leader Protease to Inhibit Host Innate Immune Response

LI Xia-ying¹, XU Wen-tao^2,3, WANG Qi-huai¹

1. Development Center of Science and Technology, Ministry of Agriculture, Beijing 100122, China;
2. College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China;
3. Supervision, Inspection & Testing Center of Genetically Modified Food Safety, Ministry of Agriculture, Beijing 100083, China

Received:2014-10-31 Online:2015-04-20 Published:2015-05-05

摘要/Abstract

摘要： 口蹄疫是由口蹄疫病毒(foot-and-mouth disease virus,FMDV)引起的一种急性、热性、高度接触传染性动物疫病.口蹄疫病毒有多种机制对抗宿主的先天性免疫应答,在这个过程中病毒的前导蛋白酶(L^pro)发挥了关键作用.L^pro可切断宿主细胞帽子依赖性的蛋白翻译,抑制干扰素蛋白的合成;L^pro通过破坏核转录因子-κB (NF-κB)的完整性或减少干扰素调节因子3/7(IRF3/7)的表达,从而抑制IFN mRNA的产生;L^pro还会参与维甲酸诱导基因Ⅰ(RIG-Ⅰ)、TANK结合激酶1(TBK1)、TNF受体相关因子3(TRAF3)和TRAF6的去泛素化,从而影响Ⅰ型干扰素信号通路的活化.

关键词: 口蹄疫病毒; 前导蛋白酶; Ⅰ型干扰素; 先天性免疫应答

Abstract: Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious animal disease caused by foot-and-mouth disease virus (FMDV). FMDV has a variety of mechanisms resisting host innate immune response, leader protease (L^pro) has played a key role in the process. L^pro can cut off the host cell cap-dependent translation, inhibiting the synthesis of interferon. L^pro through destroying the integrity of NF-κB or reducing the expression of IRF3/7, inhibits the production of interferons mRNA. Besides, L^pro can take part in the deubiquitination of reninoic-acid-inducible gene Ⅰ (RIG-Ⅰ), TANK binding kinase 1 (TBK1), TNF receptor-associate factor 3 (TRAF3) and TRAF6, thus affecting the activation of type Ⅰ interferon signaling pathway.

Key words: FMDV; leader protease; typeⅠinterferon; innate immune response

中图分类号:

S852.65

李夏莹, 许文涛, 汪其怀. 口蹄疫病毒前导蛋白酶抑制宿主先天性免疫应答的机制[J]. , 2015, 42(4): 1028-1031.

LI Xia-ying, XU Wen-tao, WANG Qi-huai. Mechanism of Foot-and-mouth Disease Virus Leader Protease to Inhibit Host Innate Immune Response[J]. , 2015, 42(4): 1028-1031.

参考文献

[1] 王莉娟,徐天刚,赵云玲,等.口蹄疫诊断技术研究进展[J].中国畜牧兽医,2005,32(3):41-44.
[2] Glaser W,Cencic R,Skern T.Foot-and-mouth disease virus leader proteinase:Involvement of C-terminal residues in self-processing and cleavage of eIF4GI[J].J Biol Chem,2001,276(38):35473-35481.
[3] Belsham G J.Influence of the Leader protein coding region of foot-and-mouth disease virus on virus replication[J].J Gen Virol,2013,94(7):1486-1495.
[4] Hinton T M,Ross-Smith N,Warner S,et al.Conservation of L and 3C proteinase activities across distantly related aphthoviruses[J].J Gen Virol,2002,83(12):3111-3121.
[5] Steinberger J,Grishkovskaya I,Cencic R,et al.Foot-and-mouth disease virus leader proteinase:Structural insights into the mechanism of intermolecular cleavage[J].Virology,2014,468-470:397-408.
[6] Chinsangaram J,Koster M,Grubman M J.Inhibition of L-deleted foot-and-mouth disease virus replication by alpha/beta interferon involves double-stranded RNA-dependent protein kinase[J].J Virol,2001,75(12):5498-5503.
[7] Brown C C,Chinsangaram J,Grubman M J.Type Ⅰ interferon production in cattle infected with 2 strains of foot-and-mouth disease virus,as determined by in situ hybridization[J].Can J Vet Res,2000,64(2):130-133.
[8] Chinsangaram J,Moraes M P,Koster M,et al.Novel viral disease control strategy:Adenovirus expressing alpha interferon rapidly protects swine from foot-and-mouth disease[J].J Virol,2003,77(2):1621-1625.
[9] 李玉娟,王殿柱,张淑霞,等.采用蚀斑技术克隆纯化口蹄疫病毒的研究[J].中国畜牧兽医,2008,35(12):71-73.
[10] Ryman K D,Klimstra W B,Nguyen K B,et al.Alpha/beta interferon protects adult mice from fatal Sindbis virus infection and is an important determinant of cell and tissue tropism[J].J Virol,2000,74(7):3366-3378.
[11] de Los S T,de Avila B S,Weiblen R,et al.The leader proteinase of foot-and-mouth disease virus inhibits the induction of beta interferon mRNA and blocks the host innate immune response[J].J Virol,2006,80(4):1906-1914.
[12] Moraes M P,Chinsangaram J,Brum M C,et al.Immediate protection of swine from foot-and-mouth disease:A combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine[J].Vaccine,2003,22(2):268-279.
[13] Honda K,Fukuhara T,Kojima Y,et al.Two cases of advanced pancreas cancer treated with GTX:Combined use of gemcitabine,docetaxel and capecitabine[J].Gan To Kagaku Ryoho,2006,33(13):2089-2092.
[14] Bonizzi G,Karin M.The two NF-kappaB activation pathways and their role in innate and adaptive immunity[J].Trends Immunol,2004,25(6):280-288.
[15] Hayden M S,Ghosh S.Keeping cartographers busy[J]. Nat Cell Biol,2004,6(2):87-89.
[16] de Los S T,Diaz-San S F,Grubman M J.Degradation of nuclear factor kappa B during foot-and-mouth disease virus infection[J].J Virol,2007,81(23):12803-12815.
[17] de Hemptinne V,Rondas D,Toepoel M,et al.Phosphorylation on Thr-106 and NO-modification of glyoxalase Ⅰ suppress the TNF-induced transcriptional activity of NF-kappaB[J].Mol Cell Biochem,2009,325(1-2):169-178.
[18] Segundo F D,Weiss M,Perez-Martin E,et al.Inoculation of swine with foot-and-mouth disease SAP-mutant virus induces early protection against disease[J].J Virol,2012,86(3):1316-1327.
[19] Wang D,Fang L,Luo R,et al.Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced type Ⅰ interferon transcription by decreasing interferon regulatory factor 3/7 in protein levels[J].Biochem Biophys Res Commun,2010,399(1):72-78.
[20] Wang D,Fang L,Li P,et al.Molecular cloning of the porcine RANTES promoter:Functional characterization of dsDNA/dsRNA response elements in PK-15 cells[J].Dev Comp Immunol,2011,35(3):345-351.
[21] Wang D,Fang L,Li P,et al.The leader proteinase of foot-and-mouth disease virus negatively regulates the type Ⅰ interferon pathway by acting as a viral deubiquitinase[J].J Virol,2011,85(8):3758-3766.
[22] Isaacson M K,Ploegh H L.Ubiquitination,ubiquitin-like modifiers,and deubiquitination in viral infection[J].Cell Host Microbe,2009,5(6):559-570.
[23] Mao A P,Li S,Zhong B,et al.Virus-triggered ubiquitination of TRAF3/6 by cIAP1/2 is essential for induction of interferon-beta (IFN-beta) and cellular antiviral response[J]. J Biol Chem,2010,285(13):9470-9476.
[24] Zhao W.Negative regulation of TBK1-mediated antiviral immunity[J].FEBS Lett,2013,587(6):542-548.
[25] Foeger N,Glaser W,Skern T.Recognition of eukaryotic initiation factor 4G isoforms by picornaviral proteinases[J].J Biol Chem,2002,277(46):44300-44309.

口蹄疫病毒前导蛋白酶抑制宿主先天性免疫应答的机制

Mechanism of Foot-and-mouth Disease Virus Leader Protease to Inhibit Host Innate Immune Response

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨苏珍, 孙亚宁, 刘运超, 柴书军, 张改平. 口蹄疫病毒感染与免疫鉴别诊断及免疫评价二联试纸的优化[J]. 中国畜牧兽医, 2022, 49(10): 3953-3962.
[2]	梁妍, 陶蕾, 宋向东, 刘宇, 赵凤舞, 李雪虎, 辛志君, 陆锡宏, 梁剑平, 冯建强, 周翔, 王震. ¹²C⁶⁺离子束辐照对茶树精油质量及其杀灭口蹄疫病毒效果的影响[J]. 中国畜牧兽医, 2021, 48(2): 755-762.
[3]	刘汉平. 猪O型口蹄疫病毒样颗粒的构建及鉴定[J]. 中国畜牧兽医, 2019, 46(11): 3350-3357.
[4]	王玉姣, 郭永丽, 罗修鑫, 高明春, 王君伟. 牛Ⅰ型干扰素受体α链与其配体结合活性的鉴定[J]. 《中国畜牧兽医》, 2018, 45(9): 2610-2619.
[5]	郑华斌, 张中旺, 吕建亮, 潘丽, 张永光. 口蹄疫病毒多基因及猪α干扰素共表达载体的构建及其免疫豚鼠效果研究[J]. 《中国畜牧兽医》, 2017, 44(5): 1452-1461.
[6]	张柳, 冯霞, 靳野, 祁淑芸, 张晓霞, 马军武. 猪免疫口蹄疫灭活疫苗后外周血细胞亚群变化的研究[J]. , 2016, 43(4): 1012-1016.
[7]	龚真莉, 蒋韬, 祁淑芸, 陈国栋, 刘艳红, 李勇. 口蹄疫病毒核酸试纸条检测方法的建立[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2292-2297.
[8]	李夏莹, 郑鹭飞, 张秀杰, 杨坤, 汪其怀, 周荣, 吴添文. 口蹄疫病毒研究进展[J]. , 2015, 42(7): 1910-1916.
[9]	解银丽, 马琪, 秦晓东, 殷相平, 柳纪省, 张志东, 李志勇. O型口蹄疫病毒衣壳酸敏感性位点的筛选[J]. , 2015, 42(4): 823-829.
[10]	刘欢欢, 谢丽君, 邵志勇, 高洪, 严玉霖. MDA5在先天性免疫抗病毒作用中的研究进展[J]. , 2015, 42(1): 230-233.
[11]	曹丽娟, 倪伟, 史慧君, 马世伟, 乔军, 陈创夫. O型口蹄疫病毒3D基因荧光定量PCR方法的建立及应用[J]. , 2014, 41(12): 34-39.
[12]	曾江勇. 口蹄疫病毒Hankou/99株基因组全序列测定与基因特征分析[J]. , 2014, 41(12): 74-80.
[13]	张庆勋, 刘新生, 周鹏, 方玉珍, 王永录, 张永光. 口蹄疫病毒病毒样颗粒研究进展[J]. , 2014, 41(11): 258-262.
[14]	宋妮, 温永俊, 王凤雪, 武华. 利用SUMO表达系统高效表达猪O型口蹄疫病毒VP0、VP1、VP3基因[J]. , 2013, 40(9): 61-65.
[15]	孙芳芳, 董英, 薛强, 邹明强. 口蹄疫病毒分子生物学检测方法研究进展[J]. , 2013, 40(6): 190-194.