O型口蹄疫病毒衣壳酸敏感性位点的筛选

doi:10.16431/j.cnki.1671-7236.2015.04.008

›› 2015, Vol. 42 ›› Issue (4): 823-829.doi: 10.16431/j.cnki.1671-7236.2015.04.008

O型口蹄疫病毒衣壳酸敏感性位点的筛选

解银丽, 马琪, 秦晓东, 殷相平, 柳纪省, 张志东, 李志勇

中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部草食动物疫病重点开放实验室, 兰州 730046

修回日期:2015-01-31 出版日期:2015-04-20 发布日期:2015-05-05
通讯作者: 李志勇 E-mail:lizhiyong02@caas.cn
作者简介:解银丽(1988-),女,河南许昌人,硕士,研究方向:分子疫苗与动物免疫学,E-mail:xieyinli98437@126.com
基金资助:
国家自然科学基金项目(31201943);国家国际科技合作专项(2011DFA31830);国家高技术研究发展计划(863)项目(2011AA10A211-3)

Selection of the Acid Sensitive Sites of FMDV Type O Capsid

XIE Yin-li, MA Qi, QIN Xiao-dong, YIN Xiang-ping, LIU Ji-xing, ZHANG Zhi-dong, LI Zhi-yong

Key Laboratory of Grazing Animal Disease of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Revised:2015-01-31 Online:2015-04-20 Published:2015-05-05

摘要/Abstract

摘要： 为筛选与O型口蹄疫病毒(FMDV)衣壳酸敏感性相关的位点,本研究以牛源O型FMDV ON株为研究对象开展耐酸性毒株筛选工作.通过给予pH 6.0酸性环境连续诱变,筛选获得6株耐酸性毒株.将诱变病毒与亲本病毒衣壳蛋白编码区P1区序列比对后发现7个核苷酸突变,即共同存在于6株耐酸毒株P1区的错义突变A1334G(Y445C)、A1643G(Q548R)、A1822G/A1823C(N608A)及同义突变G1371A,另有仅存在于耐酸毒株m2 P1区的错义突变C1849T(P617S)和仅存在于耐酸毒株m1 P1区的同义突变G468T.本研究为O型FMDV酸敏感性分子机制的揭示及病毒衣壳酸稳定性的提高奠定了基础.

关键词: O型口蹄疫病毒; 衣壳; 酸敏感性; 耐酸位点

Abstract: The study was aimed to select the acid sensitive sites of foot-and-mouth disease virus (FMDV) type O capsid. Using the bovine FMDV type O ON strain as the research object, six acid resistance monoclonal strains were obtained after treatment in pH 6.0acidic environment for serial passages. The P1 region which encoding their capsid proteins of the mutagenic viruses was sequenced and compared with the parental virus, then seven mutation sites were found, of which the mutation sites A1334G(Y445C), A1643G(Q548R), A1822G/A1823C (N608A) of P1 region found in six strains were sense mutation, while the mutation site G1371A was nonsense mutation. In addition the mutation site C1849T(P617S) of P1 region found in m2 was sense mutation and the mutation site G468T of P1 region found in m1 was nonsense mutation. This research laid a good foundation for the reveal of the acid sensitive mechanism and the acid sensitive enhance of FMDV type O capsid.

Key words: FMDV type O; capsid; acid sensitivity; acid resistance sites

中图分类号:

S855.3

解银丽, 马琪, 秦晓东, 殷相平, 柳纪省, 张志东, 李志勇. O型口蹄疫病毒衣壳酸敏感性位点的筛选[J]. , 2015, 42(4): 823-829.

XIE Yin-li, MA Qi, QIN Xiao-dong, YIN Xiang-ping, LIU Ji-xing, ZHANG Zhi-dong, LI Zhi-yong. Selection of the Acid Sensitive Sites of FMDV Type O Capsid[J]. , 2015, 42(4): 823-829.

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O型口蹄疫病毒衣壳酸敏感性位点的筛选

Selection of the Acid Sensitive Sites of FMDV Type O Capsid

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