鸭源多杀性巴氏杆菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.02.035

Abstract

Abstract: In order to identify the pathogens of clinically suspected duck Pasteurella multocida infection,in this experiment,bacteria isolated and cultured,morphological observation,biochemical identification,16S rRNA gene sequencing analysis,bacterial species-specific identification,capsule type identification and animal regression test were carried out,drug resistance was analyzed by drug sensitivity test and drug resistance gene detection.The results showed that the bacteria isolated from the liver tissue of the diseased duck showed smooth bulging and gray-white colonies on the blood agar medium,which was Gram-negative bacillus,and the Wright's staining showed two-level thick staining;Biochemical identification showed that the isolated strain could ferment glucose,sucrose and mannitol,and the test results of hydrogen sulfide,oxidase and hydrazine were positive.The phylogenetic tree analysis of the 16S rRNA gene sequence showed that the isolated strain was clustered with the genus Pasteurella,homology > 99%.The results of bacterial species specific identification were consistent with Pasteurella multocida.The results of capsule typing only amplified the target gene fragment of about 1 050 bp,which was consistent with capsule serotype A.Animal regression test showed that the isolated strain had strong pathogenicity.The drug sensitivity test showed that the isolated strain was resistant to 12 drugs such as carbenicillin,ampicillin,cotrimoxazole and tetracycline.PCR detection of drug resistance gene showed that the isolate carried Sul1,Sul3,tet(X) and Intl1 four drug resistance genes,consistent with the drug sensitivity phenotype.In this experiment,a duck capsular serotype A Pasteurella multocida was successfully isolated,which provided a reference for the prevention and treatment of duck Pasteurella multocida infection.

Key words: duck; Pasteurella multocida; isolation and identification; drug resistance

CLC Number:

S852.61

CHEN Guoquan, ZHANG Xu, YAN Chaohua, ZHOU Bijun, WANG Kaigong, CHENG Zhentao, WEN Ming. A Isolation,Identification and Drug Resistance Analysis of Pasteurella multocida from Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 620-628.

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A Isolation,Identification and Drug Resistance Analysis of Pasteurella multocida from Ducks

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