《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1508-1515.doi: 10.16431/j.cnki.1671-7236.2019.05.030

• 基础兽医 • 上一篇    下一篇

兔奇异变形杆菌的分离鉴定及耐药性分析

王俊书1, 徐进强1, 金红岩1, 侯绍华2, 封家旺1   

  1. 1. 西藏职业技术学院, 拉萨 850030;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193
  • 收稿日期:2018-11-06 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 侯绍华, 封家旺 E-mail:houshaohua@126.com;67278012@qq.com
  • 作者简介:王俊书(1991-),女,河北邯郸人,硕士,研究方向:动物微生物学,E-mail:1219227241@qq.com
  • 基金资助:

    2017年西藏自治区科研创新团队项目;西藏自治区自然科学基金项目(XZ2017ZRG-113);2018年西藏职业技术学院校级课题(2018ZR-09);中国农业科学院创新工程项目(CAAS-ASTIP-2018-IAR)

Isolation,Identification and Drug Resistance Analysis of Rabbit Proteus mirabilis

WANG Junshu1, XU Jinqiang1, JIN Hongyan1, HOU Shaohua2, FENG Jiawang1   

  1. 1. Tibet Vocational and Technical College, Lhasa 850030, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2018-11-06 Online:2019-05-20 Published:2019-05-20

摘要:

为了解拉萨市一种兔场种兔腹泻死亡的病因,本试验从一只种兔的肠道内容物中分离出一株细菌,通过革兰氏染色、生化鉴定、16S rRNA PCR扩增和序列比对、动物回归试验及药敏试验进行了分析。结果显示,分离菌株为单个或成对的短杆状的革兰氏阴性菌;分离菌与GenBank中奇异变形杆菌的同源性为73.5%~99.8%,采用Mega 7.0软件将分离菌株与11株参考菌株的16S rRNA序列进行同源性比对分析,并构建系统进化树,综合分析后确定分离菌株为奇异变形杆菌,命名为T2018。在动物回归试验中有3只试验兔出现了腹泻,但均未死亡,剖检结果显示,空肠、回肠肠壁薄而透明,内有半透明胶冻样物;结肠和盲肠黏膜充血,浆膜上有出血斑点。药敏试验显示,分离菌株仅对阿莫西林/克拉维酸、庆大霉素和喹诺酮类的氧氟沙星、环丙沙星、诺氟沙星5种抗菌药表现出高度敏感,对哌拉西林表现为中介,对其他24种抗生素均表现为耐药。上述结果表明该种兔场种兔腹泻死亡的病原为奇异变形杆菌。

关键词: 兔; 奇异变形杆菌; 分离鉴定; 耐药性; β -内酰胺酶

Abstract:

In order to understand the cause of diarrhea death in a rabbit field in Lhasa,a strain of bacterium was isolated from the intestinal contents of a rabbit,and was isolated and identified by Gram staining,biochemical identification,16S rRNA PCR amplification and sequence alignment,animal regression test and drug sensitivity test.The results showed that the isolated strain was a single or pair of short rod-shaped Gram-negative bacteria,and the homology of the isolate with Proteus mirabilis in GenBank were 73.5% to 99.8%,the homologous analysis of the 16S rRNA sequence of the isolates and 11 reference strains was carried out by Mega 7.0 software,and the phylogenetic tree was constructed.The isolated strain was identified as Proteus mirabilis,named as T2018.In the animal regression test,there were 3 cases of diarrhea,but none of them died.The results of the necropsy showed that the jejunum and ileum were thin and transparent,with translucent jelly inside;Colon and cecal mucosa were congested,there were bleeding spots on the serosa.Susceptibility tests showed that the isolated strains were only highly sensitive to the five antibacterial agents of amoxicillin/clavulanic acid,gentamicin and ofloxacin,ciprofloxacin and norfloxacin,intermediate to lacillin,and resistant to 24 other antibacterials.The above results indicated that the diarrhea death of rabbits was caused by Proteus mirabilis.

Key words: rabbit, Proteus mirabilis, isolation and identification, drug resistance, β-lactamase

中图分类号: