芽孢杆菌B13的分离鉴定及其抑菌作用研究

doi:10.16431/j.cnki.1671-7236.2019.02.029

Abstract

Abstract:

The aim of the experiment was to find a new antimicrobial bacterium,then analyze its main metabolic antimicrobial characterization.A strain was isolated from pig intestinal contents with resistance to E.coli K88,Staphylococcus aureus and Salmonella Typhimurium by gel punching.Its taxonomic status determined by colonial morphology,Gram staining and 16S rDNA sequence analysis.Then the fermentation medium and conditions for its growth and antimicrobial activity were optimized.The results showed that the isolated strain was identified as Bacillus sp.and showed an excellent antimicrobial activity against E.coli K88,Staphylococcus aureus and Salmonella Typhimurium,and then was named as Bacillus sp.B13.It was Gram-positive,single-birth or in pairs of growth.Auxanographic analysis showed that the optimal growth condition of Bacillus sp.B13 was that the pH was 6.0,temperature was 34℃,sucrose as carbon source and peptone as nitrogen source.Results of antimicrobial activity against E.coli K88,Staphylococcus aureus and Salmonella Typhimurium showed the optimal condition was that pH was 7.0,temperature was 35℃,maltose as carbon source and peptone as nitrogen source.In conclusion,Bacillus sp.B13 had good bacteriostatic activities and had great development potential applied prospects in the production.

Key words: Bacillus sp.; isolation; identification; optimization

CLC Number:

S852.61

LIU Shaona, ZHANG Bin, XIANG Decai, ZHAO Zhiyong, ZHAO Yanguang. Isolatiog,Identification and Study of a Bacillus sp.B13 with High Antibiotic Character[J]. , 2019, 46(2): 573-581.

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Isolatiog,Identification and Study of a Bacillus sp.B13 with High Antibiotic Character

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