猪链球菌4型分离株生物学特性研究

doi:10.16431/j.cnki.1671-7236.2019.01.010

Abstract

Abstract:

In order to analyze the pathogenic biological characteristics of Streptococcus suis type 4(SS4).10 strains of SS4 from different regions in China were subjected to multilocus sequence typing.Seven conserved housekeeping genes aroA,gki,dpr,mutS,recA,thrA and cpn60 of Streptococcus suis were amplified by PCR.The sequencing results were uploaded to MLST database to find the sequence type,and the clustering analysis map was constructed to clarify the genetic relationship in strains.The seven major virulence genes gdh,mrp,epf,sly,fbps,gapdh and orf2 were identified by PCR,and the distribution of virulence factors of SS4 was analyzed by virulence factor spectrum.BALB/c mice were subjected to animal pathogenicity test with 10 strains of purified bacteria,and the strongest strains were selected according to the lethal number of mice and subjected to New Zealand rabbit pathogenicity test.The results of multilocus sequence typing showed that 6 strains were ST850,3 strains were ST1006,and 1 strain was ST94.According to the location analysis,the strains in Guangdong and Jiangsu had high homology,and the strains in Jiangsu and Shanghai showed differentiation,which showed genetic diversity.gdh,gapdh and orf2 genes were detected in 10 strains, sly gene was detected in 7 strains,and fbps gene were detected in 4 strains.According to the virulence factor spectrum,a total of 3 virulence genotypes were found,6 strains were gdh⁺sly⁺gapdh⁺orf2⁺ type,3 strains were gdh⁺fbps⁺gapdh⁺orf2⁺ type,and 1 strain was gdh⁺sly⁺fbps⁺gapdh⁺orf2⁺ type.Animal pathogenicity test results showed that 10 strains could all cause the death of BALB/c mice.The LD₅₀ of SH1510 was as low as 1×10⁸ CFU,which was the most pathogenic to mice,and SH1510 could cause typical neurological symptoms and death in New Zealand rabbits.The above results provided new data for the genetic evolution and virulence studies of Streptococcus suis,which had enriched the study of Streptococcus suis.

Key words: Streptococcus suis serotype 4(SS4); MLST; virulence factor; pathogenicity

CLC Number:

S852.61⁺1

SUN Ke, NI Yanxiu, ZHU Haodan, WANG Dandan, GUAN Lin, YU Zhengyu, ZHOU Junming, LYU Lixin, WANG Chunfeng, HE Kongwang. Study on Biological Characteristics of Streptococcus suis Type 4 Isolates[J]. , 2019, 46(1): 80-88.

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Study on Biological Characteristics of Streptococcus suis Type 4 Isolates

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