2株Ⅰ群禽腺病毒的分离鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2018.12.030

Abstract

Abstract:

In order to understand the genetic evolution and pathogenicity of fowl adenovirus (FAdV) in Shandong province,we conducted two henneries suspected outbroke inclusion body hepatitis and pericardial effusion syndrome of pathogenetic materials (liver and spleen) by PCR identification,preliminary considered they were two FAdV virus strains.The positive pathogenetic materials were grinded by the extract of inoculate LMH cells,then we subculture the strain viruses.The CPE observation,PCR analysis,TCID₅₀ calculation,chicken embryos pathogenicity test,SPF chicken regression analysis,and the phylogenetic analysis of hexon gene were performed in this study.We successfully isolated two FAdV virus strains,and found that the CPE could be found in the first generation,and the PCR analysis showed the bands (500 bp) were in the target locations.The F5 TCID₅₀ was 10^-7.75/0.1 mL for the first strain and 10^-7.60/0.1 mL for the second strain,and they both had strong pathogenicity to 7 days old SPF chicken embryo.When the 21 days old SPF chickens were infected by these two strains,the death rates were 80% and 15%,respectively.The nucleotide homology analysis of hexon gene showed that the first isolate had the highest homology with FAdV-4 (99.57%),and the second isolate had the highest homology with FAdV-8b (99.18%).Both of these two strains were the group Ⅰ viruses,which were different from the hemorrhagic enteritis of turkeys viruses (in group Ⅱ) and the egg drop syndrome viruses (in group Ⅲ).The first isolate was type C in the genotype,type 4 in the serotype,and was named as FAdV-SDC4;While the second isolate was type E in the genotype,type 8b in the serotype genotype,and was named as FAdV-SDE8b.Our study indicated that both of FAdV-SDC4 and FAdV-SDE8b strains were the epidemic strains in China in recent years,and our analysis laid the basis for the management to inclusion body hepatitis and pericardial effusion syndrome,and could contribute to the vaccine research to these diseases.

Key words: fowl adenovirus Ⅰ; isolation and identification; hexon gene; pathogenicity

CLC Number:

S852.65

WANG Ying, REN Guoxin, JIN Xin, ZHANG Wanlin, ZHANG Qingxia, ZHANG Yu, YUAN Juan, YANG Wenting, JIANG Wei, ZHOU Zhencheng. Isolation,Identification and Pathogenic Analysis of Two Strains of Fowl Adenovirus Ⅰ[J]. , 2018, 45(12): 3563-3571.

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Isolation,Identification and Pathogenic Analysis of Two Strains of Fowl Adenovirus Ⅰ

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