鸽GnIH和GnRH基因克隆及其在不同繁殖阶段下丘脑中的表达

doi:10.16431/j.cnki.1671-7236.2018.09.017

Abstract

Abstract:

In order to clarify the expression levels of gonadotropin-inhibitory hormone (GnIH) and gonadotropin-releasing hormone (GnRH) genes in hypothalamus of pigeons at different reproductive stages,and explore the relationship between GnIH and GnRH genes and reproductive performance,the CDS sequence of GnIH and GnRH genes were amplified,cloned and analyzed,and the expression level of GnIH and GnRH genes was detected in hypothalamus of pigeons in pre-laying,post-laying and feeding periods,respectively.Sequence analysis results showed that 522 bp of pigeon GnIH gene complete CDS (GenBank accession No.:MG589638) was obtained which encoded 173 amino acids,and 276 bp of pigeon GnRH gene complete CDS (GenBank accession No.:MG589639) was obtained which encoded 91 amino acids.The homology between GnIH and GnRH genes of pigeon and those of other birds were more than 85% and 80%,respectively.GnIH precursor included a GnIH peptide and two GnIH-related peptides (GnIH-RP-1,GnIH-RP-2) which had the typical C-terminal "LPXRF-amide" sequence.GnRH precursor included a signal peptide,a GnRH peptide and a GnRH-associated peptide (GAP).Real-time PCR results indicated that the expression level of GnIH gene in hypothalamus of pigeons in pre-laying period was extremely significantly higher than that in post-laying and feeding periods (P<0.01).The expression level of GnRH gene in hypothalamus of pigeons in post-laying period was extremely significantly higher than that in pre-laying and feeding periods (P<0.01).The expression level of GnIH gene in hypothalamus of pigeons in pre-laying period was extremely significantly higher than GnRH gene (P<0.01).The expression levels of GnRH gene in hypothalamus of pigeons in post-laying and feeding periods were extremely significantly and significantly higher than GnIH gene (P<0.01;P<0.05).The results revealed that the expression of GnIH and GnRH genes was related to the transformation of female pigeons in different breeding stages,which laid a foundation for discussing the molecular mechanism of pigeon production.

Key words: pigeon; GnIH gene; GnRH gene; production

CLC Number:

S836

ZHANG Rui, CHANG Lingling, MU Chunyu, FU Shengyong, TANG Qingping, BU Zhu. Cloning and Expression of GnIH and GnRH Genes in Hypothalamus of Pigeons at Different Breeding Stages[J]. , 2018, 45(9): 2478-2485.

References

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Cloning and Expression of GnIH and GnRH Genes in Hypothalamus of Pigeons at Different Breeding Stages

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