催乳激素对山羊脂肪酸合酶基因转录活性的影响

doi:10.16431/j.cnki.1671-7236.2018.08.026

Abstract

Abstract:

This study was aimed to investigate the effects of lactogenic hormones on transcriptional activity of fatty acid synthase (FASN) gene in goat mammary epithelial cells cultured in vitro.The goat mammary epithelial cells were treated with different concentrations of insulin (INS),estradiol (E₂),prolactin (PRL) and hormone combinations (INS+PRL,E₂+INS+PRL) for 24 h,respectively.The relative mRNA expression of FASN gene was measured by Real-time quantitative PCR.Moreover,other cells were transfected with goat FASN gene promoter reporter vectors.Then,the cells were treated with INS,E₂,PRL and hormone combinations (INS+PRL,E₂+INS+PRL) for 24 h,respectively.The promoter activity of FASN gene was detected by dual-luciferase reporter assay system.The results showed that the promoter activity and mRNA level of FASN gene were extremely significantly increased in 10 and 100 μmol/L E₂ group (P<0.01),and also extremely significantly or significantly increased in 0.1 and 1 μg/mL PRL group (P<0.01;P<0.05).However,different concentrations of INS had no effect on the transcriptional activity of FASN gene (P>0.05).In addition,hormone combinations (INS+PRL,E₂+INS+PRL) significantly increased the promoter activity and mRNA expression level of FASN gene (P<0.05).In conclusion,E₂ and PRL could regulate the transcriptional activity of FASN gene,which provided a theoretical basis for further study of the molecular regulation mechanism of FASN gene during lactation.

Key words: goat; fatty acid synthase (FASN); lactogenic hormones; transcriptional activity

CLC Number:

S827

LI Jun, QUAN Kai, ZHANG Guizhi, ZHAO Jinyan, DENG Hongyu, HE Qiuya, SHI Huaiping. Effect of Lactogenic Hormones on the Transcriptional Activity of FASN Gene in Goats[J]. , 2018, 45(8): 2255-2262.

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Effect of Lactogenic Hormones on the Transcriptional Activity of FASN Gene in Goats

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