鹦鹉幼雏病病毒福建株VP1基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.06.030

Abstract

Abstract:

In order to characterize the VP1 gene of budgerigar fledgling disease polyomavirus (BFPyV) from Melopsittacus undulates (designated as FJ-2016 strain),specific primers were designed according to the VP1 gene of BFPyV retrieved from GenBank.The full-length sequence of VP1 gene of FJ-2016 strain was amplified by PCR.The target fragment was recovered,cloned and sequenced.The results demonstrated that the length of VP1 gene was 1 032 bp,coding 343 amino acids.The VP1 protein theoretical pI,instability index,aliphatic index and grand average of hydropathicity was 5.77,40.91(means unstable protein),74.72 and -0.366,respectively.The sequences were submitted to the GenBank,the accession No. was MG148345.Nucleotides homology of the VP1 gene of BFPyV isolates were above 99.1% with each other.The VP1 gene nucleotides comparison indicated that the VP1 gene was very conserved among different time,regions and species.Phylogenetic tree based on the VP1 gene showed that BFPyV isolates with different time and regions shared closer relationship with each other,which also could be divided into two genetic clades (Clade 1 and Clade 2).The Melopsittacus undulates origin BFPyV could be found in the two genetic clades,these data indicated that Melopsittacus undulates origin BFPyV shared no significant phylogenetic evolution relationship with the time and regions.In conclusion,this study provided evidence of budgerigar fledgling disease polyomavirus circulating in Fujian,which enriched the BFPyV molecular epidemiology data in China.

Key words: budgerigar fledgling disease polyomavirus; Melopsittacus undulatus; VP1 gene; sequence analysis

CLC Number:

S858.93

WAN Chunhe, CHENG Longfei, FU Guanghua, SHI Shaohua, CHEN Hongmei, FU Qiuling, LIU Rongchang, LIN Jiansheng, HUANG Yu. Cloning and Sequence Analysis of VP1 Gene of Budgerigar Fledgling Disease Polyomavirus from Fujian[J]. , 2018, 45(6): 1661-1667.

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Cloning and Sequence Analysis of VP1 Gene of Budgerigar Fledgling Disease Polyomavirus from Fujian

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