3个地方猪品种BOLL和STRA8基因结构变异位点的群体分布研究

doi:10.16431/j.cnki.1671-7236.2018.06.022

Abstract

Abstract:

To explore the differences of population distribution of two structural variations(BOLL-I6-SV285 from the intron 6 of BOLL gene on chromosome 15 and STRA8-I4-SV447 from the intron 4 of STRA8 gene on chromosome 18) between local pig breeds and European ones,the genotypes were detected by PCR method taking four pig breeds as samples,including Congjiang Xiang pig,Jiangkouluobo pig,Rongchang pig and Large White pig breeds.The functional elements in structural variations regions were analyzed using the online softwares of miRBase,UCSC and RegRNA 2.0.The results showed that an insertion of DNA fragment with 285 bp in length was confirmed in the structural variations of BOLL-I6-sv285,the dominant genotypes were DI and DD genotypes in three local pig breeds,and the frequency of allele I in the local pig breeds was higher than that in Large White pigs.Furthermore,Ⅱ genotype of BOLL-I6-sv285 was significantly related with higher litter size of Congjiang Xiang pig (P<0.05).For the structure variation STRA8-I4-SV447,it was an insertion mutation with 447 bp,and DD genotype was the dominant genotype in four pig groups,and the frequency of allele I in Congjiang Xiang pig,Jiangkouluobo pig and Large White pig were higher than that in Rongchang pig.The insertion types (Ⅱ) of STRA8-I4-SV447 was significantly related with lower litter size of Congjiang Xiang pig (P<0.05).This results suggested that BOLL-I6-sv285 and STRA8-I4-sv447 could be taken as candidate sites for the litter size choice in Congjiang Xiang pig population.

Key words: BOLL gene; STRA8 gene; structural variation; Congjiang Xiang pig; Jiangkouluobo pig; Rongchang pig

CLC Number:

S813.3

LI Dapeng, RUAN Yiqi, LIU Chang, NIU Xi, HUANG Shihui, RAN Xueqin, WANG Jiafu. Study on the Population Distribution of Two SV Sites in BOLL and STRA8 Genes of Three Indigenous Pig Breeds[J]. , 2018, 45(6): 1599-1607.

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Study on the Population Distribution of Two SV Sites in BOLL and STRA8 Genes of Three Indigenous Pig Breeds

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