狐源豚鼠气单胞菌M12的鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2017.08.028

Abstract

Abstract:

We tried to identify the bacteria and explore the mechanism of the bacteria's pathogenicity via housekeeping gene gyrB and in vitro organ culture (IVOC) of ileum and intramuscular injection. Microscope, electron microscope and scanning electron microscope were also used to observe and the structure, pathogen of M12 and changes of infected tissues. The results showed that M12 was short gram-negative bacteria, and both ends of it were obtuse and the size without flagellum was (0.6 to 1.6) μm×(0.6 to 0.7)μm while length of flagellum was about 2 to 3 times of the length of bacteria.It had 100.0% similarity with Aeromonas caviae from GenBank. IVOC test observed M12' adhesion in intestinal epithelial cell, resulting in formulation of biofilm structure and damage in intestinal epithelial cell. Histopathologic examination showed that M12 could cause damage in intestine, liver, lung, kidney, muscle tissue of rabbit.The assay would offer references for researching the pathogenic mechanism of Aeromonas caviae.

Key words: in vitro organ culture (IVOC); Aeromonas caviae; adhesion; identification; pathogenicity

CLC Number:

S852.61⁺2

MENG Cang-long, TONG Pan-pan, SHAO Xi-qun, CHEN Li-zhi. Identification and Pathogenicity Analysis of Aeromonas caviae M12 from Fox[J]. , 2017, 44(8): 2417-2423.

References

[1] 何永龙,黄锦炉,乔晶鑫,等. 草鱼致病性豚鼠气单胞菌的分离鉴定[J].中国预防兽医学报,2015,37(12):912-916.
[2] 陈翠珍,张晓君,房海,等.鲢鳙打印病豚鼠气单胞菌主要特性及系统发育学分析[J].中国农学通报, 2006,22(5):455-459.
[3] 龙苏,韩书煜,牛志伟,等. 胡子鲶致病性气单胞菌的分离鉴定及其致病力与毒力基因型相关性[J]. 水产学报,2016,40(3):308-317.
[4] 黄明春.从肺结核痰液中检出一株豚鼠气单胞菌[J]. 广西医科大学学报,2000,17(3):458.
[5] 鲁辛辛,南风英.从胆汁中分离出豚鼠气单胞菌[J]. 临床检验杂志,1993,11(2):108.
[6] 李婉萍,彭仕明,陈武,等. 中国豹源豚鼠气单胞菌的分子鉴定与毒力基因分析[J]. 野生动物学报,2015,36(1):29-33.
[7] Schüller S, Lucas M, Kaper J B, et al. The ex vivo response of human intestinal mucosa to enteropathogenic Escherichia coli infection[J]. Cell Microbiol,2009,11(3):521-530.
[8] 王海娟, 王利. 鲤鱼杀鲑气单胞菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医,2015,42(1):192-196.
[9] Asakura H, Hashii N, Uema M, et al. Campylobacter jejuni pdxA affects flagellum-mediated motility to alter host colonization[J]. PLoS One,2013,8(8):e70418.
[10] 翟新新,温振才,沈雪飞,等. 维氏气单胞菌鞭毛蛋白flaA的克隆及原核表达[J]. 中国畜牧兽医,2014,41(7):40-43.
[11] 朱春红,董立伟,张江英,等. 鞭毛素fliC基因的缺失对肠炎沙门氏菌生物膜形成的影响[J].中国预防兽医学报,2016,49(4):417-422.
[12] 董立伟. 肠炎沙门氏菌鞭毛素fliC基因缺失突变株的构建及相关功能性分析[D]. 扬州:扬州大学,2014.
[13] Chua K L, Chan Y Y, Gan Y H. Flagella are virulence determinants of Burkholderia pseudomallei[J]. Infect Immun,2003,71(4):1622-1629.
[14] Kirov S M, Castrisios M, Shaw J G. Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces[J]. Infect Immun,2004,72(4):1939-1945.
[15] Chellappa S T, Maredia R, Phipps K, et al. Motility of Pseudomonas aeruginosa contributes to SOS-inducible biofilm formation[J]. Res Microbiol,2013,164(10):1019-1027.
[16] Lai S, Tremblay J, Déziel E. Swarming motility:A multicellular behaviour conferring antimicrobial resistance[J]. Environ Microbiol,2009,11(1):126-136.
[17] 孟菲,梨书长,杨武宁,等. 铜绿假单胞菌生物膜研究进展[J]. 中国畜牧兽医,2016,43(11):2976-2981.
[18] 丁莉莎,王瑶.鞭毛介导的运动性与细菌生物膜的相互关系[J]. 微生物学报,2009,49(4):417-422.
[19] Best A, La Ragione R M, Clifford D, et al. A comparison of Shiga-toxin negative Escherichia coli O157 a flagellate and intimin deficient mutants in porcine in vitro and in vivo models of infection[J]. Vet Microbiol,2006,113(1-2):63-72.
[20] Donlan R M. Biofilm elimination on intravascular catheters:Important considerations for the infectious disease practitioner[J]. Clin Infect Dis,2011,52(8):1038-1045.
[21] Knutton S, Lloyd D R, Candy D C, et al. Adhesion of enterotoxigenic Escherichia coli to human small intestinal enterocytes[J]. Infect Immun,1985,48(3):824-831.
[22] Dos Santos P A, Pereira A C, Braga R L, et al. Adhesion and cytotoxicity of Aeromonas caviae to rabbit intestinal epithelium ex vivo[J]. Antonie Van Leeuwenhoek,2016,109(9):1261-1270.
[23] 吉莉莉. 南方鲇溃疡综合症病原菌分离、鉴定与病理形态学研究[D]. 雅安:四川农业大学,2008.
[24] 贺扬, 华丽, 汪开毓,等. 高致病性维氏气单胞菌胞外产物对斑点鲖的致病性[J].水产学报,2016,40(3):457-467.

Identification and Pathogenicity Analysis of Aeromonas caviae M12 from Fox

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	PENG Yangxiang, YANG Zhenbiao, YAN Kuiyou, WANG Han, HU De, WANG Zun, LIU Ning, ZHAO Liansheng. From Artificial to Intelligent:Research Progress of Individual Idendification Technology for Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1855-1866.
[3]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[4]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[5]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[6]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[7]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[8]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[9]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[10]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[11]	XIAO Yangyang, LI Ruirui, MA Zhongchen, TANG Tian, WANG Na, CHEN Chuangfu, ZHENG Wei, WANG Yong, WANG Pengyan. Isolation,Identification and Drug Sensitivity Analysis of Mycoplasma bovis in Shihezi Region of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 754-763.
[12]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[13]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[14]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[15]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.