不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究

doi:10.16431/j.cnki.1671-7236.2017.02.033

Abstract

Abstract:

In order to understand the molecular mechanism of host susceptibility differential to highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSV) infection among three breeds of pig, Tibetan pig,ZangMei Black and Yorkshire,the mRNA level of five reported PRRSV receptor genes (HSPG2,SIGLEC1,CD163,VIM and NMMHC-ⅡA) expressed in lung were investigated on 0,4, 7 and 14 days post infection (dpi) with HP-PRRSV JXA1 isolate.The Real-time RT-PCR results showed that the expression of HSPG2 in Tibetan pig increased significantly on 4 and 14 dpi and decreased significantly on 7 dpi compared with the expression on 0 dpi (P < 0.05), and its expression in ZangMei Black on 14 dpi was significantly higher than that of on 7 dpi (P < 0.05);An increased expression of SIGLEC1 in Tibetan pig was observed on 4 and 14 dpi (P < 0.05), and a decreased expression of the same gene was detected in Yorkshire on 4,7 and 14 dpi (P < 0.05);The expression of CD163 elevated significantly on 14 dpi in both Tibetan pig and ZangMei Black (P < 0.05), however its expression reduced significantly on 7 and 14 dpi in Yorkshire (P < 0.05); The expression of VIM gene was significantly higher on 7 dpi in Tibetan pig and significantly lower on 7 dpi in Yorkshire (P < 0.05); And the expression of NMMHC-ⅡA was significantly higher on 4 dpi in ZangMei Black, which was also significantly higher in Yorkshire on 4 and 14 dpi (P < 0.05).In conclusion, SIGLEC1 and VIM might be important genes to influence the host resistance of Tibetan pig,ZangMei Black and Yorkshire to HP-PRRSV infection.

Key words: JXA1 isolate; Tibetan pig; ZangMei Black; Yorkshire; PRRSV receptor genes; differential expression

CLC Number:

S855.3

KANG Run-min, JI Gao-sheng, ZENG Kai, LV Xue-bin, YIN Ming-yu. Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV[J]. , 2017, 44(2): 538-545.

References

[1] Hopper S A,White M E,Twiddy N.An outbreak of blue-eared pig disease (porcine reproductive and respiratory syndrome) in four pig herds in Great Britain[J].Vet Rec,1992,131(7):140-144.
[2] Lager K M,Halbur P G.Gross and microscopic lessions in porcine fetuses infected with porine reporductive and respiratory syndrome virus[J].J Vet Diagn Invest,1996,8(3):275-282.
[3] loula T.Mystery pig disease[J].Agri-practice,1991,12(1):23-24.
[4] Cho J C,Dee S A.Porcine reproductive and respiratory syndrome virus[J].Theriogenology, 2006,66(3):655-662.
[5] 李彬,王豪男,彭忠,等.华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析[J].中国畜牧兽医,2015,42(11):3026-3036.
[6] 王振华,曹肖涵,宋禾,等.猪繁殖与呼吸综合征病毒的危害及免疫策略[J].中国畜牧兽医,2014,41(2):239-243.
[7] Halbur P G,Rothschild M F,Thacker B J,et al.Differences in susceptibility of Duroc, Hampshire, and Meishan pigs to infection with a high virulence strain (VR2385) of porcine reproductive and respiratory syn-drome virus (PRRSV)[J].Journal of Animal Breeding and Genetics,1998,115(3):181-189.
[8] Jiang C,Xing F,Xing J,et al.Different expression patterns of PRRSV mediator genes in the lung tissues of PRRSV resistant and susceptible pigs[J].Dev Comp Immunol,2013,39(1-2):127-131.
[9] Liang W,Li Z,Wang P,et al.Differences of immune responses between Tongcheng (Chinese local breed) and Large White pigs after artificial infection with highly pathogenic porcine reproductive and respiratory syndrome virus[J].Developmental & Comparative Immunology,2016,215:84-93.
[10] Kang R M,Ji G S,Yang X,et al.Investigation on host susceptibility of Tibetan pig to infection of porcine reproductive and respiratory syndrome virus through viral challenge study[J].Veterinary Microbiology,2016,183:62-68.
[11] Shi C,Liu Y,Ding Y,et al.PRRSV receptors and their roles in virus infection[J].Arch Microbiology,2015,197(4):503-512.
[12] Tian K,Yu X,Zhao T,et al.Emergence of fatal PRRSV variants:Unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark[J].PLoS One,2007,2(6):e526.
[13] Zhou Y S,Yang X,Wang H Y,et al.Molecular characterization of a complete genome and 12 Nsp2 genes of PRRSV of Southwestern China[J].Food Environ Virol,2012,4(3):102-114.
[14] Uddin M J,Cinar M U,Tesfaye D,et al.Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues[J].BMC Res Notes,2011,4:441.
[15] Delputte P L,Costers S,Nauwynck H J.Analysis of porcine reproductive and respiratory syndrome virus attachment and internalization: Distinctive roles for heparan sulphate and sialoadhesin[J].J Gen Virol, 2005,86(5):1441-1445.
[16] Delputte P L,Vanderheijden N,Nauwynck H J,et al.Involvement of the matrix protein in attachment of porcine reproductive and respiratory syndrome virus to a heparinlike receptor on porcine alveolar macrophages[J].J Virol,2002,76(9):4312-4320.
[17] Breedam W V,Gorp H V,Zhang J,et al.The M/GP(5) glycoprotein complex of porcine reproductive and respiratory syndrome virus binds the sialoadhesin receptor in a sialic acid-dependent manner[J].PLoS Pathog,2010,6(1):el000730.
[18] Ait-Ali T,Wilson A D,Carré W,et al.Host inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-Ⅰinterferon transcriptional response[J].Immunogenetics,2011,63(7):437-448.
[19] Calvert J G,Slade D E,Shields S L,et al.CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses[J].J Virol,2007,81(14):7371-7379.
[20] Kim J K,Fahad A M,Shanmukhappa K,et al.Defining the cellular target(s) of porcine reproductive and respiratory syndrome virus blocking monoclonal antibody 7G10[J].J Virol,2006,80(2):689-696.
[21] Zhang H,Guo X,Ge X,et al.Changes in the cellular proteins of pulmonary alveolar macrophage infected with porcine reproductiveand respiratory syndrome virus by proteomics analysis[J].J Proteome Res,2009,8(6):3091-3097.
[22] Zhou E M,Xiao Y,Shi Y,et al.Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus[J].J Virol Methods,2008,149(2):300-308.
[23] Rey M,Valenzuela-Fernández A,Urzainqui A,et al.Myosin ⅡA is involved in the endocytosis of CXCR4 induced by SDF-1alpha[J].J Cell Sci,2007,120(6):1126-1133.

Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	NIE Jingru, MA Li, YAN Dawei, DENG Jun, ZHANG Hao, ZHANG Bo, LIU Jinqiao, DONG Xinxing. Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2855-2868.
[3]	XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.
[4]	ZHAI Yaying, SHI Qiaoting, CHU Qiuxia, ZHU Xiaoting, HUA Liushuai, ZHANG Zijing, CHEN Fuying, QI Xinglei, WANG Eryao, LYU Shijie. Differential Expression Analysis of Plasma Exosomes microRNA Between Donor and Recipient Cattle Before Embryo Transfer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 641-649.
[5]	LI Yueyue, LYU Dongling, LIU Xiaoxiao, TIAN Weilong, LIANG Liang, CHEN Kuirong, CHENG Feng, LAN Ganqiu, LIANG Jing. Bioinformatics Analysis and Tissue Expression of ZnT Gene Family in Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4119-4128.
[6]	LI Huihui, GAO Xuefeng, XU Ke, ZHAO Baokai, WANG Ying, SHI Lijun, WANG Lixian. Influencing Factors Analysis and Genetic Parameters Estimation of Gestational Length in Yorkshire Sows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4261-4269.
[7]	ZHAO Yuqiang, CHEN Xin, LI Qingchun, MA Jilin, DONG Yinhe, WANG Deming, GUO Lingyun, HUANG Tao. Polymorphism of ENO3 Gene and Its Correlation with Growth Traits in Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 188-196.
[8]	MA Li, NIE Jingru, LU Shaoxiong, LIU Shiyi, WANG Lin, LI Jintian, YAN Dawei, DONG Xinxing. Comparison of the Taste Activity Value of Full Spectrum Free Amino Acid in Muscle of Diqing Tibetan Pig and Wild Boar×Diqing Tibetan Pig [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1275-1283.
[9]	LI Qianwen, YANG Zhen, YANG Yanan, WANG Zhengwen, WEN Yuliang, CAI Yuan, ZHAO Shengguo. Effects of Environmental Temperature in Winter and Summer on Physiological Characteristics and Related Gene Expression of Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 873-881.
[10]	ZENG Maoqin, LIU Yanhan, ZHANG Qiandong, BI Wenwen, YE Ni, WANG Yizhou, YANG Ying, CHENG Zhentao, WEN Ming. Differential Analysis of miRNA Expression Profiles in Liver of Duck Infected with Duck Enteritis Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4170-4181.
[11]	ZHAO Xialing, XIAO Qi, QIAN Wenxian, WEN Dongxu, YAN Mingshuai, LYU Lixin, NIU Jiaqiang, HE Kongwang. Isolation,Identification and Biological Characteristics of Staphylococcus chromogenes from Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 3014-3021.
[12]	LI Wufeng, SUN Yutong, ZHAO Jingwei, GUAN Jiawei, DU Min. Cloning,Sequence Analysis and Differential Expression of HSL Gene in Guangling Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2348-2358.
[13]	HUANG Liangzong, ZHANG Hailong, YAN Guangzhi, XIE Bo, CHEN Shengnan, DENG Rusen, GU Wanjun. Study on the Regulation of microRNA-124-3p on Lung Injury in Mice Infected with H1N1 Subtype Swine Influenza Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3049-3057.
[14]	WANG Zhenmei, DUAN Mengqi, LU Fenghui, WANG Shixin, QIANGJIUZHUOMA, TAN Zhankun, ZHANG Jian, SHANG Peng. Polymorphism and Tissue Expression Patterns of CKMT2 Gene in Tibetan and Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3305-3313.
[15]	TIAN Qin, ZHANG Minghua, ZHOU Bijun, CHENG Zhentao, WANG Kaigong, WEN Ming. Screening of Duodenal Mucosal Inflammation Related Genes and Regulatory Pathways in Ducks with Duck Plague Virus Infection [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3323-3333.