辽宁地区奶牛乳房炎细菌性病原分离鉴定及大肠杆菌药敏试验

doi:10.16431/j.cnki.1671-7236.2016.11.040

Abstract

Abstract:

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.

Key words: dairy cow mastitis; pathogenic bacteria; E.coli; antibiotic sensibility

CLC Number:

S852.61

YAO Wei, YU Xue-wu, CAO Dong, ZHAO Xiao-tong, GU Gui-bo, CHEN Yao, ZHANG Lei, LIU Yao-chuan, FENG Jun-ke. Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli[J]. , 2016, 43(11): 3080-3084.

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Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

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