China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (3): 178-182.

• 疾病防治 • Previous Articles     Next Articles

Full Length Analysis of NDV Epidemic Strain GX-08 and its Pathogenicity Experiment to Duck

WANG Jun-feng1，CAI Li-li1，WU Zhi-xin1，GONG Zhong-gui1，HUANG Xing-guo2，CAO Wei-sheng1，HUANG Shu-jian2   

  1. （1.College of Veterinary Medicine，South China Agricultural University，Guangzhou 510642，China；2.College of Life Science，Foshan University，Foshan 528231，China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: HUANG Shu-jian；CAO Wei-sheng

Abstract: Newcastle disease virus strain GX-08，isolated from the invasive immunized chicken flocks in Guangxi province in 2008，was velogenic according to the pathogenetic tests and highly pathogenicity to duck. According to the sequences of NDV strains published in GenBank，ten pairs of primers were designed and complete genome cDNA of the isolate was successfully amplified by PT-PCR. Sequence analysis showed that the genome length of GX-08 strain was 15192 nt which contained six ORFs，encoding 6 proteins of NP，P，M，F，HN and L，respectively. The cleavage motif 112R-R-Q-K-R-F117 of F protein was identical to the characteristic of velogenic strain. The evolution analysis of the 47 to 420 nt sequence including the cleavage site of F gene indicated that GX-08 strain belonged to genotype Ⅶ.

Key words: Newcastle disease virus; pathogenicity test; complete genome; phylogenetic analysis

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