牛源大肠杆菌O157:H7的分离鉴定

doi:10.16431/j.cnki.1671-7236.2016.10.040

Abstract

Abstract:

The objective of this study was to investigate the infection and pathogenicity of E.coli O157:H7 from 564 fecal samples in three parts of Xinjiang,such as Akesu,Yili and Tacheng.Suspected strains were detected by biochemical and PCR after enrichment by EC broth,being chosen by SMAC plate and screened by MUG test.On the basis of this,the isolated strains were used in mice attack drug test.The results showed that there were two strains of E.coli O157:H7 (named Y166 and Y226,respectively) were isolated from the collected samples of Yili,and the detection rate was 0.88%.In the mice attack drug test,the mice of Y166 and Y226 groups were all died within 48 h.In conclusion,two strains of E.coli O157:H7 were isolated and identified with pathogenic from samples in Yili area,and no E.coli O157:H7 were identified in the samples from Akesu and Tacheng.

Key words: E.coli O157:H7; isolation and identification; PCR; pathogenicity

CLC Number:

S852.61

WANG Yu-meng, ZHANG Tao, DING Hao, QI Wen-Jing, HE Guo-wen, SU Zhan-qiang, KUANG Ling. Isolation and Identification of Escherichia coli O157: H7 from Cattle[J]. , 2016, 43(10): 2780-2785.

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Isolation and Identification of Escherichia coli O157: H7 from Cattle

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