›› 2011, Vol. 38 ›› Issue (3): 132-134.

• 遗传繁育 • Previous Articles     Next Articles

Polymorphisms in Exons of Porcine Erythropoietin Receptor Gene

ZHANG Long-chao,LI Wen,ZHAO Ke-bin,LI Yong,WANG Li-gang,YAN Hua,WANG Li-xian   

  1. (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: WANG Li-xian

Abstract: Polymorphisms were screened in exons of porcine erythropoietin receptor(EPOR) gene by resequencing exons using PCR amplification of a DNA pool from 5 Beijing
Black pigs and 5 Min pigs. 3 SNPs, g.1147C>G in exon 2, g.1645G>A in exon 3 and g.4953C>T in exon 8, were detected. In which, g.1147C>G in exon 2 causes an amino acid substitution (Leu>Val) at position 50 (NP_999457), and g.1645G>A in exon 3 causes an amino acid substitution (Arg>His) at position 92. The third SNP, g.4953C>T in exon 8, was a synonymous varibation. Except for g.4953C>T, Hardy-Weinberg equilibriums were found at g.1147C>G and g.1645G>A in 30 Beijing Black pigs.

Key words: pig; EPOR gene; exon; polymorphism

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