利用Solexa测序技术鉴定策勒黑羊microRNA

doi:10.16431/j.cnki.1671-7236.2016.01.030

Abstract

Abstract: With the purposes of providing basic information for further studies of Qira Black sheep reproduction activities such as folliculogenesis and hormone secretion, characterization of microRNA(miRNA) expression in Qira Black sheep ovary tissues was studied.In this study, the small RNA isolated from total RNA of Qira Black sheep ovary tissues were sequenced by Solexa and then bioinformatics analysis were performed.The results showed that 9527311 clean reads were obtained, and the 21-23 nt small RNA was the major RNA in the total sequences.434 ovary conservative miRNA and 57 new miRNA were identified.Among them, there were 167 conservative including 8 new miRNA exceeded 100 in the expression levels.The study succeeded to construct the expression library of miRNA which were abundant and differentially expressed in Qira Black sheep ovary tissues.

Key words: miRNA; Solexa; ovary; Qira Black sheep

CLC Number:

ZENG Xian-cun, JIA Bin, SHI Hong-cai, SHEN Hong, ZHANG Yong-sheng, CHEN Han-ying. microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing[J]. , 2016, 43(1): 204-209.

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microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing

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