水貂、狐狸和貉源犬瘟热病毒F蛋白信号肽区基因克隆及遗传变异分析

doi:10.16431/j.cnki.1671-7236.2015.04.010

Abstract

Abstract: To investigate the prevalence and genetic diversity of canine distemper virus (CDV) in the main breeding areas of fur animal in China in recent years, the tissue samples of the minks, foxes and raccoon dogs with symptoms of canine distemper (CD) were collected from Shandong, Hebei and Liaoning provinces in the years 2012 to 2014.The signal peptide of fusion protein (Fsp) genes of 15 CDV wild-type strains were cloned and sequenced after the samples were identified to be positive of CD by one step CDV rapid test kit and RT-PCR. The results showed that the identities of nucleotide and amino acid sequences of the 15 CDV wild-type strains were 93.6% to 100.0%, while the identities of nucleotide and amino acid sequences of the isolates and vaccines were 80.7% to 81.7%. The phylogenetic analysis of Fsp genes revealed the 15 CDV wild-type strains were characterized as an Asia-1 genotype that were circulating currently in China. The alignment analysis of amino acid sequences indicated there were obviously diversity at the amino acid sites 3 to 14, 16 to 37, 47 to 67.The sequences analysis of Fsp gene of the currently circulating CDV wild-type strains suggested this Fsp gene region had high divergence and could be used to identify the CDV genotypes that would provide foundation for the prevention and control of CD in the fur animal.

Key words: mink; fox; raccoon dog; canine distemper virus; signal peptide of fusion protein (Fsp)

CLC Number:

S852.65

SUN Yan-gang, ZHAO Jian-jun, BAI Xue, NIU Deng-yun, SHAO Xi-qun, HU Bo, LIU Hao, ZHANG Hai-ling, ZHANG Lei, WANG Yang, MA Fan-shu, YAN Xi-jun. Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs[J]. , 2015, 42(4): 838-846.

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Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs

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