奶牛乳房炎金黄色葡萄球菌8种毒力基因的PCR检测

Abstract

Abstract: The aim of the present study was to investigate the main virulence genes properties of Staphylococcus aureus (SA) strains from bovine mastitis.For this purpose,8 kinds of virulence genes were detected from 70 isolates of clinical mastitis and 55 isolates of subclinical mastitis with PCR.The results were as follows,the detecting rates of nuc,ClfA,TSST-1,PVL,Hla,Hlb,FnBPA and FnBPB were 100.0% (70 isolates),100.0% (70 isolates),0 (0 isolate),5.7% (4 isolates),100.0% (70 isolates),11.4% (8 isolates),97.1% (68 isolates) and 100.0% (70 isolates) respectively in clinical mastitis;whereas 100.0% (55 isolates),100.0% (55 isolates),0 (0 isolate),70.9% (39 isolates),98.2% (54 isolates),9.1% (5 isolates),100.0% (55 isolates) and 100.0% (55 isolates) in subclinical mastitis. The results implied that nuc,ClfA,Hla,Hlb and FnBPA were the main virulence genes in SA isolates both in clinical mastitis and subclinical mastitis. PVL gene might be a key pathogenic gene for the cows with subclinical mastitis.

Key words: bovine mastitis; Staphylococcus aureus (SA); virulence genes; PCR

CLC Number:

XIE Xiu-lan, LIU Xi-yuan, WANG Jian-dong, FAN Chun-mei, YANG Hui-jun, KANG Xiao-dong. Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis[J]. , 2014, 41(10): 47-50.

References

1 马保臣,柴同杰,秦卓明,等.用PCR与ELISA、RPLA对照检测乳腺炎金黄色葡萄球菌肠毒素和毒素休克综合症素-1[J].中国兽医学报,2008,28(8):901~905.
2 王琴,杨丽美,骆方军.金黄色葡萄球菌耐药性及 mecA、qacA/B 和 pvl基因检测[J].中华临床感染病杂志,2009,2(2):102~105.
3 王新,韦艺媛,张静,等.乳房炎奶牛金黄色葡萄球菌毒素基因的检测及PFGE分型研究[J].畜牧兽医学报,2011,42(7):974~980.
4 苏艳,王世民,邵俊高,等.牛源金黄色葡萄球菌黏附素纤连结合蛋白A分泌型DNA疫苗诱导小鼠免疫效应的研究[J].中国畜牧兽医,2013,40(2):27~30.
5 李丽,刘代成,杨宏军,等.多重PCR检测奶牛乳腺炎金黄色葡萄球菌粘附素基因clfA、clfaB、fnbpA和fnbpB[J].生物学杂志,2011,28(3):55~57.
6 杨波.金黄色葡萄球菌毒力基因检测的研究[D].乌鲁木齐:新疆农业大学,2009.
7 迟佳琦,李闰婷,朱战波,等.奶牛乳房炎金黄色葡萄球菌毒力因子与荚膜基因的PCR检测[J].中国预防兽医学报,2009,31(11):860~864.
8 张静,于三科,王新,等.原料乳和临床乳房炎金黄色葡萄球菌毒力基因检测及药敏分析[J].中国兽医学报,2012,32(5):759~764.
9 夏胜超,吴聪明,王绍琛,等.奶牛乳房炎病例中金黄色葡萄球菌毒素基因的检测[J].中国兽医杂志,2007,43(4):23~24.
10 Brakstad O G,Aasbakk K,Maeland J A. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene[J].J Clin Microbiol,1992,30(7):1654~1660.
11 Isrina S,Salasia O,Khusnan Z,et al.Comparative studies on phenol-and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany[J].Journal of Veterinary Science,2004,5(2):103~109.
12 Jarraud S,Cozon G,Vandenesch F,et al.Involvement of enterotoxins G and I staphylococcal toxin shock syndrome and staphylococcal scarler fever[J].J Clin Microbiol,1999,37(8):2446~2449.
13 Kalorey D R,Shanmugam Y,Kurkure N V,et al.PCR-based detection of genes encoding virulence determinants in Staphyloccous aureus from bovine subclinical mastitis cases[J].Journal of Veterinary Science,2007,8(2):151~154.
14 Karahan M,Acik M N,Cetinkaya B.Invitigation of toxin genes by polymerase chain reaction in Staphycoccus aureus strains isolated from bovine mastitis in Turkey[J].Foodborne Pathog Dis,2009,6(8):1029~1035.
15 Oliveira L,Rodriques A C,Hulland C,et al.Enterotoxin production,enterotoxin gene distribution,and genetic diversity of Staphyloccous aureus recovered from milk of cows with subclinical mastitis[J].AmJ Vet Res,2011,72(10):1361~1368.
16 Peles F,Wagner M,Varga L,et al.Characterization of Staphylococcus aureus stains isolated from bovine milk in Hungary[J].Int J Food Microbiol,2007,118(2):186~193.
17 Ünal N,Askar Ş,Macun H C,et al.Panton-Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of Staphyloccocci isolated from milks of small ruminats[J].Trop Anim Health Prod,2012,44(3):573~579.

[1]	WANG Zhifang, XU Yindi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, LI Haili, WANG Keling. Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3245-3255.
[2]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[3]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[4]	DONG Hongyan, SONG Haigang, ZHU Shanyuan, YU Shengqing, XIE Jun, HONG Shuangpeng, GUO Changming, XU Hai, GAO Yuexiu. Isolation,Identification and Pathogenicity Analysis of Escherichia coli from Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1977-1984.
[5]	LIANG Qian, BAO Taotao, XIAN Simei, YANG Qian, LI Pengfei, GU Qinglin, ZHENG Weihao, DU Peng, QING Chengxin. Establishment of PMA-PCR Method for Detection of Orf Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1524-1531.
[6]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[7]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[8]	MAI Zhanhai, LU Yabin, LI Jianlong, ZHAO Lu, SUN Danlan, TONG Panpan, KUANG Ling. Effects of Honeysuckle, Forsythia and Their Drug Pair on Resistance Genes and Virulence Genes of Streptococcus equi subsp. equi [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 709-717.
[9]	JIA Tong, ZHANG Di, ZHANG Zhaotian, CHEN Panliang, ZHANG Jie, LI Yinghui, ZHU Yue, QIN Jianhua, LI Yan. Detection of Virulence and Resistance Genes and Drug Sensitivity Analysis of Escherichia coli Derived from Diarrheic Calves in Beijing-Tianjin-Hebei Partial Regions [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4820-4831.
[10]	ZHANG Su, FANG Yuwen, CHEN Yunsheng, GAO Li, XU Guiyun, ZHU Kui, ZHENG Shijun, WANG Yongqiang. Isolation,Identification and Antibiotic Resistance Analysis of Bacillus cereus on Eggshell [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4476-4484.
[11]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[12]	QU Zhehui, ZHANG Xiwen, ZHAO Yu, ZHENG Quanfang, LIAN Huixiang, ZHAO Yunhuan, GUO Xiaoqiu. Genetic Variation Analysis of Major Virulence Genes of Pseudorabies Virus Epidemic Strains in Southern Henan Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3019-3029.
[13]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[14]	YANG Xiaobin, HE Lili, ZHANG Yang, ZHOU Ruifeng, HOU Kangli, ZHANG Yunyun, WANG Yueli, LI Qingqing, SHI Bingang, HU Jiang. Polymorphisms of LF Gene and Their Association with Milk Quality Traits in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1655-1663.
[15]	GE Qiang, MA Dongxin, ZHOU Yuqing, CHEN Huaijun, YUAN Jingzhi, LI Xun, WANG Xiaoye. Isolation,Identification and Biological Characteristics of Proteus mirabilis of Swine [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1804-1815.

Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments