超声波提取牛奶中细菌DNA方法的优化

Abstract

Abstract: All dairy cows’ milk were collected from Urumqi Yanerwo cow farm in May 2013.Treated those samples with different ultrasonic power （0，100，120，140，160 W） after mixed，then extracted the milk bacterial DNA with the CTAB and SDS methods，respectively，according the DNA concentration, purity and bacterial universal primers of 16S rDNA V6-V8 region to judging the quality of DNA，finally screen out the best DNA extraction method.The results showed that the DNA which was extracted by the CTAB and SDS methods after treated with ultrasonic power 0 W for 10 min couldn’t amplify the V6-V8 of 16S rDNA.Meanwhile，the V6-V8 of 16S rDNA could been amplified with the other ultrasonic power (100，120，140，160 W)，and the result showed multifariously.The highest DNA density was 203.35 μg/mL，which was extracted by the CTAB method and treated with ultrasonic power 140 W.Results indicated that using the CTAB method after treated those samples with ultrasonic power 140 W was the best way to get the significantly higher DNA density，this method could further satisfy the requirements of studying about microbial populations of mastitis from the molecular level.

Key words: mastitis; bacterial; ultrasonic wave; DNA extraction; polymerase chain reaction

YAN Xiao-fei，LIN Shan，TONG Ting，WANG Jin-quan，HUANG Ke-xin，DONG Xuan，YUE Mei-ting，YE Dong-dong，YANG Bin. Improvement of Ultrasonic Power on DNA Extraction Method from Milk Bacterial[J]. .

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Improvement of Ultrasonic Power on DNA Extraction Method from Milk Bacterial

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