Abstract: Herein, aflatoxin M₁(AFM₁) was analysed on a conventional HPLC by a chromatography column packed with 2.6 μm core-shell particles and large volumn injection technique. The results show that the core-shell column is faster and more effective than typical total porous particles packed column even on conventional HPLC. With the same instrumental condition and weak injection solvent, the analysis time of new 2.6 μm core-shell particle column (4.6 mm×100 mm) is 51.5% and 21.3% of tranditional total porous 3 μm particle(4.6 mm×150 mm) and 5μm particle(4.6 mm×250 mm) columns respectively. Meanwhile, the peak height is 183.9% and 490.5% of the latter columns and the peak variances is 34.7% and 6.2% of the latter columns. This indicates that the use of core-shell columns on conventional HPLC leads to the reductions of analysis time and cost with increased sensitivity and accuracy.

Key words: HPLC; core-shell column; large volumn injection; aflatoxin M₁