隆林山羊肌细胞生成素基因的序列分析与结构预测

Abstract

Abstract: To enrich the basical genetic information and explore the sequence structure and the regulation mechanism of muscle differentiation in local excellent variety Longlin goat MyoG gene. A pair of primers was designed to amplify the sequence of MyoG gene from Longlin goat genome. The results showed that the sequence of MyoG gene with 2419 bp consisted of 3 exon, 2 intron and some of 5'and 3'untranslated regions. With 675 bp coding sequences, encoding a total of 224 amino acids, and the peptide chain had not signal peptide and transmembrane structure.Homology analysis showed, comparing the coding sequences and coding amino acids of Longlin goat MyoG with other species, the cluster result was similar to the phylogenetic tree. Both showed that Longlin goat and sheep, cattle, wild boar were closest relative, the homology of amino acid sequences between them was above 95%. So the mammalian MyoG gene was conservative in evolution, and cloning, sequence analysis and structure prediction of Longlin goat MyoG gene provided the biological information and theoretical basis for researching the gene expression and regulation, improving the muscle quality and developing goats breed.

Key words: Longlin goat; MyoG gene; sequence analysis; structure prediction

CLC Number:

ZHAO Zi-gui, HUANG Chen, SONG Shao-rui, CAO Zhi-zhi, GUO Ya-fen, LAN Gan-qiu, YANG Ying-bai. Sequence Analysis and Structure Prediction of MyoG Gene in Longlin Goat[J]. , 2012, 39(11): 17-21.

References

1 王立新,白俊杰,叶星,等.草鱼 MyoD cDNA 的克隆和序列分析[J].中国农业科学,2005,38(10):2134~2136.
2 刘铮铸,巩元芳,张传生,等.波尔山羊MyoG基因的鉴定和序列分析[J].畜牧兽医学报,2010,41(10):1337~1341.
3 刘铮铸,巩元芳,张文香,等.山羊MyoG基因启动子区序列分析与结构预测[J].畜牧兽医学报,2012,43(1):29~36.
4 吴海.隆林县发展隆林山羊现状及前景分析[J].广西畜牧兽医,2011,27(4):235~236.
5 宋兴超,杨福合,邢秀梅,等. 甘肃马鹿MyoG基因5'UTR单核苷酸多态性检测及其序列变异分析[J]. 中国畜牧兽医, 2010, 37(9): 100~105.
6 林万华,黄路生.猪肌肉细胞生成素基因的研究进展[J].国外畜牧杂志,2000,27(1):29~30.
7 高勤学,刘梅.猪MyoG基因的PCR-RFLP分型及其生长性能和肌纤维数目的相关性分析[J].中国兽医学报,2005,25(5):330~332.
8 高光宇,王杰,王永,等.成都麻羊MyoG基因的克隆与测序分析[J].西南民族大学学报,2009,25(1):84~88.
9 Ernst C W,Mendez E A,Robic A,et al.Rapid communication:myogenin (MYOG) physically maps to porcine chromosome 9q2.1-q2.6[J]. J Anim Sci,1998,76(1):328.
10 Hasty P,Bradley A,Morris J H,et al.Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene[J]. Nature,1993,364:502~506.
11 Malik S,Huang C F,Sehmidt J.The role of the CANNTG promoter element(E box) and the myocyte-enhancer-binding-factor-2 (MEF-2) site in the transcriptional regulation of the chick myogenin gene[J]. Eur J Biochem,1995,230(1):88~96.
12 Nabeshima Y K,Hanaok A K,Hayasaka M,et al.Myogenin gene disruption results in perinatal lethality because of severe muscle defect[J]. Nature,1993,364:532~534.
13 Rudnicki M A,Jaenisch R.The MyoD family of transcription factors and skeletal myogenesis[J]. Bioessays,1995,17:204~208.
14 Soumillion A,Erkens J H,Lenstra J A,et al.Genetic variation in the porcine myogenin gene locus[J]. Mamm Genome,1997,8(8):563~565.
15 Tseng B S,Cavin S T,Hoffman E P,et al.Human bHLH transcription factor gene myogenin(MYOG):genomic sequence and negative mutation analysis in patients with severe congenital myopathies[J]. Genomies,1999,57(3):418~421.
16 Wright W E,Sassoon D A,Lin V K.Myogenin,a factor regulating myogenesis,has a domain homologous to MyoD[J]. Cell,1989,56:203~208.
17 Wei Q,Paterson B M.Regulation of MyoD function in the dividing myoblast[J]. FEBS Lett,2001,490:173~177.

[1]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[2]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[3]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[4]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[5]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[6]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[7]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[8]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[9]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[10]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[11]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[12]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.
[13]	YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.
[14]	PAN Pengcheng, WEN Binhua, XIE Wan, JIANG Changjin, JIAO Di, CHEN Baojian, GUAN Zhihui, XIE Bingkun. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression Analysis of PDK4 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2337-2347.
[15]	CAO Yanhong, XUAN Zeyi, CHEN Baojian, WU Zhuyue, HUANG Mingguang, ZHAO Linling, ZHOU Heng, LUO Menghe, WEI Mingsong, MO Liuzhong, DENG Hongying, HUANG Haihui, CHEN Shaomei. Study on the Effect of Hybridization of Longlin Goats and Nubia Goats on Growth Performance and Meat Quality of Offspring [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2133-2141.

Sequence Analysis and Structure Prediction of MyoG Gene in Longlin Goat

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments