反向遗传学技术及其在口蹄疫病毒研究中的应用

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (1): 138-140.

• 疾病防治 • Previous Articles Next Articles

Reverse Genetic Technique and its Application in Foot-and-mouth Disease Virus

ZHU Xiu-mei¹, MU Hong-tao¹, HUANG An-cang², HUANG Yin-jun¹, WEI Guang-sen¹

（1.China Agricultural Veterinary Biological Science and Technology Co.Ltd，Lanzhou 730046， China;2.Qingyang Animal Health Inspection in Gansu Province, Qingyang 745000， China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: WEI Guang-sen

Abstract

Abstract: Reverse genetics is a new developed molecular biology technique. It can run a variety of modify and transform on DNA level for virus genome，and then these genetic manipulation effect is judged by phenotype change of rescued virus. So it can be used to research the expression and regulation mechanism of virus genome and molecular mechanism of virus pathogenicity. The technique has been applied in foot-and-mouth disease virus research，the purpose, methods and applications were also reviewed.

Key words: foot-and-mouth disease virus; reverse genetic; full-length cDNA

CLC Number:

S852.65⁺9.6

ZHU Xiu-mei;MU Hong-tao;HUANG An-cang;HUANG Yin-jun;WEI Guang-sen. Reverse Genetic Technique and its Application in Foot-and-mouth Disease Virus[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(1): 138-140.

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Reverse Genetic Technique and its Application in Foot-and-mouth Disease Virus

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