猪繁殖与呼吸综合征病毒强、弱毒株Nsp9基因的反向遗传学替换改造

doi:10.16431/j.cnki.1671-7236.2016.11.007

Abstract

Abstract:

The Nsp9 gene of porcine reproductive and respiratory syndrome virus（PRRSV）coded the RNA polymerase,which played an important role in the virus replication.In order to determine the interaction between the Nsp9 gene and virulence,replication capacity and cell tropism,the Nsp9 gene sequence of PPRSV vaccine strain CH-1R was cloned,and ligated to a virulent strain XH-GD infectious clone after restriction enzyme digestion,and transformed the virus under the basis of the reverse genetics.The results showed that the virus was successfully saved.And the results of biological characteristics test showed that titers and growth rate of saved virus were obviously higher than that of parental strain rXH-GD,but were lower than vaccine strain CH-1R.While plaques of two strains were similar in size.The polymerase of vaccine strain CH-1R could help to improve the drop degree of the virus,and this test could lay the foundation for further study of the effect of Nsp9 gene on virus virulence.

Key words: PRRSV; Nsp9 gene; reverse genetics; virus salvation

CLC Number:

Q785

ZHAO Meng-meng, QIAN Juan-juan, CUI Tian-tian, FENG Song-lin, WANG Wen-jia, XING Xing, FENG Jia-ping, ZHANG Gui-hong. Substitution between Virulent and Avirulent PPRSV Vaccine Strains with Reverse Genetics System[J]. , 2016, 43(11): 2852-2858.

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Substitution between Virulent and Avirulent PPRSV Vaccine Strains with Reverse Genetics System

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