藏猪源粪肠球菌的分离鉴定及耐药基因与毒力基因检测

doi:10.16431/j.cnki.1671-7236.2025.04.041

Abstract

Abstract: 【Objective】 This study was aimed to isolate and identify Enterococcus faecalis and determine the status of drug resistance and virulence genes in samples collected from Tibetan pigs in Linzhi,Tibet,and provide a certain reference for the pathogenic diagnosis of enterococcosis in Tibetan pigs.【Method】 Bacterial isolation and culture,Gram staining,biochemical experiments,Ef0027 specific gene PCR identification,16S rRNA identification were performed on samples collected from Tibetan pigs,and the drug resistance and virulence genes of the isolated strains were detected by PCR method.【Result】 The isolated bacteria appeared brown black colonies on PSE agar medium,suspected to be Enterococcus faecalis.Gram staining microscopy showed that the isolated bacteria appeared as single or double oval shaped cocci.The biochemical identification results showed that the isolated bacteria were negative for arabinose, rhamnose and raffinose, while they were positive for glucose, sorbitol, sucrose, lactose, arginine dihydrolase and mannitol. PCR amplification results showed that an Ef0027 gene band with a size of approximately 518 bp was obtained.A band with a size of approximately 1 500 bp was amplified from 16S rRNA gene,and the similarity with the sequence of Enterococcus faecalis registered in NCBI was greater than 98%.Eight strains of Enterococcus faecalis were isolated and identified in this study.tetB,catⅠ,gyrA,bla_TEM-1,aac3 and SUL-Ⅰ resistance genes were detected in all 8 strains,while fosA7 gene was not detected.The results of virulence gene detection showed that Esp,EfaA,GelE and Agg genes were detected in all the 8 strains,the detection rate of CylA gene was 87.5%,and no Ace gene was detected.【Conclusion】 In this study,8 strains of Enterococcus faecalis of Tibetan pig origin were isolated,all of which had multiple drug resistance genes and virulence genes.The results of this study could provide some reference for the etiological diagnosis of Enterococcus faecalis disease from Tibetan pigs.

Key words: Enterococcus faecalis; isolation and identification; drug resistance gene; virulence gene

CLC Number:

S852.61⁺1

HE Yuxuan, CIRING Zhuoma, WANG Yu, LIU Huaizhi, YANG Jinpeng, WEI Mingbang, SHANG Peng. Isolation and Identification of Tibetan Pig-derived Enterococcus faecalis and Detection of Drug Resistance and Virulence Genes[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1895-1904.

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Isolation and Identification of Tibetan Pig-derived Enterococcus faecalis and Detection of Drug Resistance and Virulence Genes

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