鸡源携带多黏菌素耐药基因mcr-1大肠杆菌耐药性与毒力基因相关性分析

doi:10.16431/j.cnki.1671-7236.2025.03.040

Abstract

Abstract: 【Objective】 The purpose of this study was to analyze the correlation between drug resistance and virulence genes of Escherichia coli with polycolistin resistance gene mcr-1 from chickens in Fujian province,and study the plasmid transfer characteristics of mcr-1 resistance gene.【Method】 The sensitivity of 87 strains of chicken Escherichia coli with mcr-1 gene to 11 kinds of antibiotics was detected by microbroth dilution method.PCR was used to detect the drug resistance gene,virulence gene and plasmid type of the isolates,and to analyze the drug resistance phenotype and drug resistance gene of mcr-1 gene positive bacteria,and the correlation between drug resistance gene and virulence gene.Plasmid conjugation test was used to investigate the transfer of mcr-1 gene.【Result】 The results of drug susceptibility test showed that 96.55% (85/87) isolates were multi-drug resistant bacteria,among which the resistance to tetracyclines was the most serious,and the resistance rates of doxycycline and tetracycline were 88.51% (77/87) and 82.76% (72/87),respectively.11 drug resistance genes were detected in the isolates,among which tetracyclines resistance gene tet(A) had the highest detection rate of 95.40% (83/87).A total of 18 virulence genes were detected,among which invasiin-type mat was the dominant virulence gene,with a detection rate of 85.06% (74/87).Some resistance genes and phenotypes,resistance genes and virulence genes were correlated.The isolates carried 12 germplasm,and the main type was IncFIB (77.01%,67/87).The conjugation test results showed that 42 isolates were successfully conjugated,and the conjugation transfer rate was 48.28% (42/87).IncI2,IncHI2 and IncX4 plasmids were successfully transferred to conjugons,and the detection rate of IncI2 plasmid was the highest (57.14%).【Conclusion】 The 87 strains of Escherichia coli with mcr-1 gene from chicken had severe multiple drug resistance,high detection rate of drug resistance genes,various types of virulence genes,and some of the drug resistance genes were correlated with drug resistance phenotype and virulence genes.The mcr-1 gene of 48.28% (42/87) strains could be transmitted by plasmids IncI2,IncHI2 and IncX4.The results provided a scientific basis for further understanding the multi-drug resistance,virulence characteristics and transmission mechanism of mcr-1 gene positive Escherichia coli from chickens in Fujian province.

Key words: Escherichia coli; polycolistin; mcr-1 gene; drug resistance; virulence gene; conjugation test

CLC Number:

S853.74

DAI Tingting, CHEN Haiyu, DUAN Chuchu, LIU Rongchang, LI Yurong, YAN Shuhan, CHEN Mengshi, LIU Luwei, BAO Yinli, CHENG Yanqing, LIN Weiming, HUANG Cuiqin, ZHENG Xintian. Correlation Analysis of Drug Resistance and Virulence Genes of Escherichia coli with Polycolistin Resistance Gene mcr-1 from Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1393-1404.

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Correlation Analysis of Drug Resistance and Virulence Genes of Escherichia coli with Polycolistin Resistance Gene mcr-1 from Chickens

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