大口黑鲈体表溃疡病原菌的分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2025.02.038

Abstract

Abstract: 【Objective】 An outbreak of disease occurred in a Micropterus salmoides aquaculture facility in Gao’an city,Jiangxi province.The diseased fish exhibited ulcers on the body surface and nodules in organs such as the liver,spleen and kidney,leading to subsequent mortality.The aim of this study was to isolate and characterize the pathogenic bacteria that caused ulcers on the body surface as well as nodules on the internal organs of Micropterus salmoides and evaluate the sensitivity of different antibacterials to this pathogen,so as to provide a scientific basis for the effective prevention and control of Micropterus salmoides ulcer disease. 【Method】 The diseased Micropterus salmoides from the aquaculture facility were dissected,and pathogenic bacteria were isolated from organs such as the liver,spleen,kidney and intestine,followed by Gram staining.The isolated strains underwent physiological and biochemical characterization,16S rDNA sequence identification,phylogenetic tree construction,artificial infection tests,histopathological examination,virulence gene detection,and antibiotic susceptibility testing. 【Result】 A dominant strain was isolated from the kidney nodules of the diseased Micropterus salmoides and named GALS1.The isolated strain formed round,smooth-surfaced colonies with regular edges and was identified as Gram-negative.The strain exhibited positive reactions for enzyme activity,glucose fermentation,nitrate reduction,and ammonia production,while methyl red and denitrification were negative,consistent with the biochemical characteristics of Aeromonas veronii (A.veronii).The 16S rDNA sequence of strain GALS1 clustered with A.veronii,showing 99% similarity,thus identified as A.veronii.Artificial infection test indicated that strain GALS1 was highly pathogenic to Micropterus salmoides,causing symptoms such as reduced vitality,surface bleeding,and hepatosplenomegaly.Virulence gene detection results showed that the strain carried virulence genes act,exu, fla and aerA.Drug sensitivity test results showed that strain GALS1 was sensitive to cefoperazone,tetracycline,gentamicin and ciprofloxacin,but resistant to penicillin,erythromycin and midecamycin. 【Conclusion】 The etiology of ulcerative and nodular disease in Micropterus salmoides at the aquaculture facility was identified as A.veronii.These findings provided reference for the prevention and control of Micropterus salmoides diseases caused by A.veronii.

Key words: Micropterus salmoides; Aeromonas veronii; identification; drug susceptibility

CLC Number:

S852.61

DU Jinhui, ZHANG Haixin, ZHANG Yanping, CHEN Wenjing, LI Wanbo, XU Xiandong. Isolation and Identification of Pathogen of Surface Ulcer in Micropterus salmoides and Drug Susceptibility Analysis[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 890-900.

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Isolation and Identification of Pathogen of Surface Ulcer in Micropterus salmoides and Drug Susceptibility Analysis

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