1株乌鳢水泡病毒的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2025.02.031

Abstract

Abstract: 【Objective】 This study was aimed to clarify the pathogen,which caused mass mortality of snakehead fish in Nanyang city,Henan province,in September 2021. 【Method】 This study identified pathogens through methods such as surface detection,microscopic observation,bacterial isolation,PCR detection,phylogenetic tree analysis,cell culture,transmission electron microscopy observation,artificial infection,and pathological section observation. 【Result】 No noticeable lesions and blooding points was observed on the surface of diseased fish.No parasite in the gills or on the body surfaces was found,and no pathogenic bacterium was isolated.PCR detection result showed that the diseased fish was positive for Snakehead fish vesiculovirus (SHVV).The similarity analysis of SHVV G gene sequence showed that the isolate had the highest similarity with the Foshan isolate HSHRV-MS2021,reaching 99.85%.The phylogenetic tree showed that the isolated strain was clustered into the same branch as SHVV-2019,SHVV-YL01 and HSHRV-MS2021 strains,belonging to the Siniperhavirus genus,and was most closely related to the HSHRV-MS2021 strain.The tissue homogenate of diseased fish could cause obvious cytopathic effect in EPC and GS cells.Transmission electron microscopy observations showed that abundant bullet shaped virus particles scattered on the surface of diseased EPC cells.Artificial infection experiments showed that infected fish exhibited symptoms similar to those of naturally diseased fish,with a mortality rate as high as 100%.Observation of tissue slices showed that the microvilli in the intestinal tract of the fish with recurrent infection disappeared,and the hemolymph in the liver and spleen increased. 【Conclusion】 In this study,a strain of SHVV was isolated from snakehead fish cultured outside Guangdong province and named SHVV-NY01.A large number of deaths of snakehead fish in this farm were caused by SHVV.The results of this study provided a reference for the epidemiological investigation and control of this virus.

Key words: snakehead fish; Rhabdovirus; Snakehead fish vesiculovirus; isolation; identification

CLC Number:

S852.65

LI Yuanyuan, YUAN Qian, FENG Xiaoran, DENG Si, LI Na, YAO Lunguang, QIN Yinghui. Isolation and Identification of a Strain of Snakehead Fish Vesiculovirus[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 811-822.

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Isolation and Identification of a Strain of Snakehead Fish Vesiculovirus

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