一株孔雀源奇异变形杆菌全基因组测序及毒力与耐药性分析

doi:10.16431/j.cnki.1671-7236.2024.07.028

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the virulence,drug resistance and genomic characteristics of Proteus mirabilis PM19 isolated from blue peacock.【Method】 Animal experiments,drug sensitivity tests and PCR methods were used to detect the virulence,drug resistance and virulence genes of the isolated bacteria.The Illumina NovaSeq6000 platform was used to sequence the genome frame diagram,and the functional genes,pathogenic host interaction protein,carbohydrate active enzyme,drug resistance gene and virulence factor prediction were carried out by NR,KEGG,Swiss-Prot,COG,PHI-base,CAZy,CARD and VFDB databases.【Result】 The isolate PM19 was a multidrug-resistant bacterium and was resistant to 14 antimicrobial agents including ampicillin,amoxicillin/clavulanate potassium,ceftriaxone,cefotaxime,amikacin,gentamicin,kanamycin,ciprofloxacin,tetracycline,doxycycline,sulfadiazine sodium,tilmicosin,florfenicol and chloramphenicol.The isolated bacteria had strong virulence,and the median lethal dose to mice was 6.19×10⁶ CFU.Eight virulence genes including FliL,zapA,rsmA,hmpA,mrpA,atfA,pmfA,ureC were detected in the strain.The whole genome sequencing analysis showed that the genome size of the strain was about 3.98 Mb,the GC content was 38.94%,and 3 715 coding genes were predicted.158 protein genes related to pathogen-host interaction were annotated by PHI-base database.101 carbohydrate active enzyme genes were annotated by CAZy database.92 drug resistance genes and 8 virulence-related genes were annotated by CARD database and VFDB database,respectively.【Conclusion】 The Proteus mirabilis strain PM19 was virulent and multidrug-resistant,carrying a large number of pathogenic host interaction protein genes,carbohydrate active enzyme genes,drug resistance genes and virulence genes.

Key words: Proteus mirabilis; multi-drug resistant; virulence; whole genome sequencing

CLC Number:

S852.61⁺2

JIAO Fengchao, LEI Zhen, LI Yingxiao, WU Xian, QU Zhehui. Whole Genome Sequencing and Virulence and Drug Resistance Analysis of a Strain of Proteus mirabilis Derived from Peacock[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3030-3040.

References

[1] SANCHES M S,BAPTISTA A A S,DE SOUZA M,et al.Proteus mirabilis causing cellulitis in broiler chickens[J].Brazilian Journal of Microbiology,2020,51(3):1353-1362.
[2] MOBLEY H L T.Proteus mirabilis overview[J].Methods in Molecular Biology,2019,2021:1-4.
[3] FLORES-MIRELES A L,WALKER J N,CAPARON M,et al.Urinary tract infections:Epidemiology,mechanisms of infection and treatment options[J].Nature Reviews Microbiology,2015,13(5):269-284.
[4] 张利锋,李娟,卢金星.奇异变形杆菌耐药性研究进展[J].疾病监测,2016,31(5):427-432. ZHANG L F,LI J,LU J X.Progress in research of antibiotic resistance of Proteus mirabilis[J].Disease Surveillance,2016,31(5):427-432.(in Chinese)
[5] WANG J T,CHEN P C,CHANG S C,et al.Antimicrobial susceptibilities of Proteus mirabilis:A longitudinal nationwide study from the Taiwan surveillance of antimicrobial resistance (TSAR) program[J].BMC Infectious Diseases,2014,14:486.
[6] 李偲,郭双,王永强,等.一株牛源都柏林沙门氏菌的全基因组测序及毒力与耐药性分析[J].微生物学通报,2023,50(6):2569-2581. LI C,GUO S,WANG Y Q,et al.Whole-genome sequencing,virulence,and drug resistance of a bovine-derived strain of Salmonella enterica subsp. enterica serovar Dublin[J].Microbiology China,2023,50(6):2569-2581.(in Chinese)
[7] 葛强,马东鑫,周雨晴,等.猪源奇异变形杆菌的分离鉴定及特征分析[J].中国畜牧兽医,2021,48(5):1804-1815. GE Q,MA D X,ZHOU Y Q,et al.Isolation,identification and biological characteristics of Proteus mirabilis of swine[J].China Animal Husbandry&Veterinary Medicine,2021,48(5):1804-1815.(in Chinese)
[8] SUN Y,WEN S,ZHAO L,et al.Association among biofilm formation,virulence gene expression,and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in Northeast China[J].BMC Veterinary Research,2020,16(1):176.
[9] XIE X,ZHANG J,WANG H N,et al.Whole genome sequence of a New Delhi metallo-β-lactamase 1-producing Proteus mirabilis isolate SNYG35 from broiler chicken in China[J].Journal of Global Antimicrobial Resistance,2021,24:266-269.
[10] 陈赫威,马玲,秦树英,等.产AmpC酶的猪源奇异变形杆菌分离鉴定及生物学特性分析[J].畜牧与兽医,2023,55(7):46-52. CHEN H W,MA L,QIN S Y,et al.Isolation,identification and biological characteristics of swine-sourced Proteus mirabilis producing AmpC enzyme[J].Animal Husbandry&Veterinary Medicine,2023,55(7):46-52.(in Chinese)
[11] 王涛,马轲,张建刚,等.长春地区鸡源奇异变形杆菌的分离鉴定及毒力分析[J].微生物学通报,2022,49(12):4987-4998. WANG T,MA K,ZHANG J G,et al.Isolation,identification,and virulence analysis of a Proteus mirabilis strain from chicken in Changchun[J].Microbiology China,2022,49(12):4987-4998.(in Chinese)
[12] 刘盼盼,贺泂杰,徐国伟,等.一株种鸡源奇异变形杆菌的分离鉴定及毒力基因分析[J].中国兽医科学,2021,51(10):1287-1294. LIU P P,HE J J,XU G W,et al.Isolation,identification and virulence gene analysis of Proteus mirabilis from chicken[J].Chinese Veterinary Science,2021,51(10):1287-1294.(in Chinese)
[13] 张健鹏,纪言霏,许丹丹,等.一株驴源奇异变形杆菌的分离鉴定与毒力基因检测[J].黑龙江畜牧兽医,2022,24:118-123. ZHANG J P,JI Y F,XU D D,et al.Isolation,identification and virulence gene detection of a strain of Proteus mirabilis from donkeys[J].Heilongjiang Animal Science and Veterinary Medicine,2022,24:118-123.(in Chinese)
[14] NORDMANN P,NAAS T,POIREL L.Global spread of carbapenemase-producing Enterobacteriaceae[J].Emerging Infectious Diseases,2011,17(10):1791-1798.
[15] ZHU X,ZHANG Y,SHEN Z,et al.Characterization of NDM-1-producing carbapenemase in Proteus mirabilis among broilers in China[J].Microorganisms,2021,9(12):2443.
[16] ARMBRUSTER C E,MOBLEY H L T,PEARSON M M.Pathogenesis of Proteus mirabilis infection[J].EcoSal Plus,2018,8(1):10.
[17] 陈小敏,杨华,桂国弘,等.奇异变形杆菌毒力因子的研究进展[J].微生物学杂志,2019,39(1):109-114. CHEN X M,YANG H,GUI G H,et al.Advances in virulence factors of Proteus mirabilis[J].Journal of Microbiology, 2019,39(1):109-114.(in Chinese)
[18] STICKLER D,GANDERTON L,KING J,et al.Proteus mirabilis biofilms and the encrustation of urethral catheters[J].Urological Research,1993,21(6):407-411.
[19] RANJBAR-OMID M,ARZANLOU M,AMANI M,et al.Allicin from garlic inhibits the biofilm formation and urease activity of Proteus mirabilis in vitro[J].FEMS Microbiology Letters, 2015,362(9):fnv049.
[20] SHI X,LIN Y,QIU Y,et al.Comparative screening of digestion tract toxic genes in Proteus mirabilis[J].PLoS One,2016,11(3):e0151873.
[21] BARBOUR E K,HAJJ Z G,HAMADEH S,et al.Comparison of phenotypic and virulence genes characteristics in human and chicken isolates of Proteus mirabilis[J].Pathogens and Global Health,2012,106(6):352-357.
[22] ZUNINO P,GEYMONAT L,ALLEN A G,et al.Virulence of a Proteus mirabilis ATF isogenic mutant is not impaired in a mouse model of ascending urinary tract infection[J].FEMS Immunology and Medical Microbiology,2000,29(2):137-143.
[23] PELLEGRINO R,SCAVONE P,UMPIÉRREZ A,et al.Proteus mirabilis uroepithelial cell adhesin (UCA) fimbria plays a role in the colonization of the urinary tract[J].Pathogens and Disease,2013,67(2):104-107.
[24] ABBY S S,CURY J,GUGLIELMINI J,et al.Identification of protein secretion systems in bacterial genomes[J].Scientific Reports,2016,6:23080.
[25] SANGERMANI M,HUG I,SAUTER N,et al.Tad pili play a dynamic role in Caulobacter crescentus surface colonization[J].Microbiology and Molecular Biology Reviews,2019,10(3):e01237-19.
[26] MERZ A J,SO M,SHEETZ M P.Pilus retraction powers bacterial twitching motility[J].Nature,2000,407(6800):98-102.
[27] PEARSON M M,SEBAIHIA M,CHURCHER C,et al.Complete genome sequence of uropathogenic Proteus mirabilis,a master of both adherence and motility[J].Journal of Bacteriology,2008,190(11):4027-4037.
[28] LI X,RASKO D A,LOCKATELL C V,et al.Repression of bacterial motility by a novel fimbrial gene product[J].The EMBO Journal,2001,20(17):4854-4862.
[29] MOBLEY H L,HAUSINGER R P.Microbial ureases:Significance,regulation,and molecular characterization[J].Microbiological Reviews,1989,53(1):85-108.

Whole Genome Sequencing and Virulence and Drug Resistance Analysis of a Strain of Proteus mirabilis Derived from Peacock

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Yan, WEI Zhitong, HU Yehao, ZHANG Huaju, ZHANG Man, FU Tong, LIU Xian, LI Zhigang, QI Xinglei, WANG Eryao, ZHANG Zijing, LIANG Dong, GAO Tengyun. Genetic Diversity and Population Structure Analysis of Jiaxian Red Cattle Based on Whole Genome Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2933-2942.
[2]	WANG Zi, SUN Miao, WANG Yongqiang, MENG Linghao, GENG Chao, SHI Jinchuan, CHEN Wei, QIU Jun, LIU Kai. Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2585-2596.
[3]	YU Fuxin, WU Siyu, YU Jieshi, ZHANG Xiaoai, YU Ting, DONG Bo, LI Jianbo, WANG Lei, WEI Wenkang. Isolation,Identification,Whole Genome Sequencing and Genetic Evolution Analysis of Goose Astrovirus in Some Areas of Guangdong Province [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2633-2643.
[4]	DU Dongdong, QIAN Jing, CHANG Hengrui, LI Hongmin, WEI Xiangli, LIU Changyong, LUO Ruifeng, WANG Guohong, KANG Lichao. Whole Genome Sequencing Analysis of a ST515 Strain of Multidrug-resistant Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2707-2716.
[5]	QIAN Ruixuan, LI Nan, KANG Lichao, MA Xun, LIU Caixia, SHI Weidi, KOU Lijun, REN Huijie, QI Yatao, YIN Zhongke, LIU Lu, WANG Jing, WANG Zhengrong, JIANG Jianjun. Influence of Coexistence of LIPI-3 and LIPI-4 on Virulence of Pathogenic Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2027-2036.
[6]	DAI Lu, ZHANG Qian, WAN Jiajia, XIE Tingting, JIA Yizhen, ZHANG Rui, ZHANG Fuxian, LIU Feng, LEI Liancheng. Isolation,Identification and Biological Characteristics Analysis of a Strain of Haemophilus parasuis Serotype 10 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1671-1685.
[7]	WANG Yingping, ZHAO Jingxian, SHANG Jiafu, HAO Chengsen, ZUO Yuqi, ZHU Huanyi, FENG Xudong, XU Tingting, NI Xingwei, LIU Xia, YANG Xiaowei, CAO Lanyun, ZHANG Liwu, TAN Changfan, CAO Lijing, ZHAO Guangwei. Isolation,Identification and Whole Genome Analysis of Aeromonas caviae from Micropterus salmoides [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1706-1716.
[8]	LIU Chunhong, LYU Zhihang, ZHANG Yuqian, LIAN Chunyang, ZHENG Xucan, YAN Changyan, ZHANG Xuelian. Isolation and Identification of Chicken-derived Salmonella Senftenberg and Detection of Drug Resistance and Virulence Genes [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1773-1783.
[9]	ZHANG Lei, CHEN Liang, JIN Zhenhua, FENG Wanyu, MIAO Yan, LAN Shijie, QIN Pingwei, SHEN Sisi, LI Dan, ZHANG Bei, DONG Jiaqiang, HUANG Xuankai, LI Xuye, JIANG Botao, WANG Hongbao, SHI Tongrui. Isolation, Identification and Pathogenic Characteristics of One Pasteurella multocida Isolate from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 347-356.
[10]	SONG Xiangbin, ZHAO Xiaoyu, LI Chuanpu, MEN Xiaodong, LIANG Meng, WEI Xiuli, LI Youzhi, YANG Zhikun, ZHANG Dechen, GUO Yuqiu, TANG Wenli. Molecular Epidemiological Analysis of Avian Extended Spectrum β-lactamases Producing Escherichia coli in Shandong Province [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 407-416.
[11]	ZI Jiaxin, LIAN Yingjie, CHEN Minyi, LI Qiuxu, ZHANG Weize, LU Mengke, ZHANG Yangyang, QU Haoxuan, MA Jun, BAI Aiquan. Drug Resistance and Pathogenicity of 4 Strains of Streptococcus suis Type 2 in Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3740-3751.
[12]	SUN Yue, WANG Qi, MAO Wei, WANG Bo, DONG Haiyan, FAN Hongliang, HAO Puguo, ZHAO Hongxia. Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3811-3822.
[13]	ZOU Hong, LUO Gan, CHEN Ling, DU Pengfei, ZHANG Qianyi, YANG Chenghuai, TIAN Ye. Research Progress on HPI Virulence Island and Its Horizontal Transfer in Pathogenic Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3430-3437.
[14]	SONG Yue, SU Shengjie, ZHANG Fan, BAI Fan, DAI Lingli, WANG Na, WANG Dawei, YANG Qianwen, ZHAO Shihua, ZHANG Yuemei. Prokaryotic Expression and Polyclonal Antibody Preparation of Mannheimia haemolytica Truncated Leukotoxin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2479-2486.
[15]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.