新吉细毛羊和小尾寒羊差异表达miRNAs筛选与调控网络分析

doi:10.16431/j.cnki.1671-7236.2023.09.004

Abstract

Abstract: 【Objective】 This study was aimed to screen the differential microRNAs (miRNAs) in Xinji Fine wool sheep (XFWS) and Small-tailed Han sheep (SXW),and study the regulatory mode of miRNAs and target genes on the mechanism expression of wool fiber.【Method】 Two kinds of Xinji Fine wool sheep and Small-tailed Han sheep with significant differences in capillary were selected,exosomes in plasma were collected for transcriptome sequencing,libraries were constructed and miRNAs were identified,miRNAs were differentially expressed using edgeR packs,miRanda and Targetscan were used to predict miRNA target genes,GO function and KEGG pathway enrichment of target genes were performed,miRNAs-mRNA interaction regulatory network for differential miRNAs target genes were analyzed,and miRNAs in transcriptome data were validated using Real-time quantitative PCR.【Result】 There were 1 019 miRNAs in the plasma exosomes of Xinji Fine wool sheep and Small-tailed Han sheep,including 112 known miRNAs,907 newly predicted miRNAs,and 364 differentially expressed miRNAs after screening,including 62 genes positively correlated with wool fineness and 302 negatively correlated genes.After multi-database comparison,18 996 annotated target genes were obtained,and GO function enrichment analysis showed that 17 193 annotated target genes were enriched into 6 608 biological processes.KEGG pathway enrichment analysis was enriched in 276 pathways,and 5 miRNAs and 42 target genes related to the expression of wool fiber mechanism were found by protein interaction analysis.Real-time quantitative PCR verified differential miRNAs,the expression of oar-miR-218a,oar-miR-370-3p,oar-miR-133,oar-miR-29a,oar-miR-27a,oar-miR-485-3p,oar-miR-22-3p,oar-miR-23a,oar-miR-148a and oar-miR-412-3p in Xinji Fine wool sheep were significantly higher than those of Small-tailed Han sheep (P<0.05), the expression of oar-miR-26a,oar-miR-29b,oar-miR-329b-3p,oar-miR-409-3p and oar-miR-30a-3p in Small-tailed Han sheep were significantly higher than those of Xinji Fine wool sheep (P<0.05),which were consistent with transcriptome sequencing results.【Conclusion】 There were 364 differential miRNAs in Xinji Fine wool sheep and Small-tailed Han sheep,including oar-miR-218a,oar-miR-370-3p,oar-miR-133,etc.,among which differential miRNAs,oar-miR-133,oar-miR-150,oar-miR-127,oar-miR-23a,oar-miR-370-3p and their 42 target genes were involved in the mechanism expression of wool fiber,FGFR2,NOTCH1 and SHH participated in multi-channel regulation and played an important role in joint regulation.

Key words: Xinji Fine wool sheep; Small-tailed Han sheep; plasma; exosomes; miRNAs

CLC Number:

ZHANG Xinyu, WANG Dan, YANG Xia, FU Jiaqi, CAO Yang, ZHANG Lichun, SUN Fuliang. Differentially Expressed miRNAs Screening and Regulatory Network Analysis of Xinji Fine Wool Sheep and Small-tailed Han Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3480-3489.

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Differentially Expressed miRNAs Screening and Regulatory Network Analysis of Xinji Fine Wool Sheep and Small-tailed Han Sheep

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