牦牛产色葡萄球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2023.07.041

Abstract

Abstract: 【Objective】 This experiment was aimed to investigate the pathogenicity and drug resistance of Staphylococcus chromogenes isolated from yaks in Lhasa, Tibet, so as to provide some reference for the prevention and treatment of Staphylococcus chromogenes infection in yaks.【Method】 Bacterial isolation and culture, biochemical test identification, 16S rDNA sequencing and similarity comparison analysis were used to identify Staphylococcus chromogenes from 65 yak samples collected from Lhasa, Tibet, and to study their drug resistance and pathogenicity.【Result】 A total of 9 isolates were obtained from 65 samples, which were named XZ1-XZ9 and the isolation rate was 13.85%.All the isolates showed small yellow colonies on 5% sheep blood plate and were positive by Gram staining.Biochemical test showed that the isolated strains were positive for mannose, fructose, sucrose and nitrate reduction, and the results were consistent with the characteristics of Staphylococcus chromogenes.Through 16S rDNA sequencing and similarity comparison analysis, it was found that the 9 isolates had the highest homology with Staphylococcus chromogenes, which was more than 96%.The results of drug sensitivity test showed that 9 strains were most resistant to three antibiotics and at least resistant to one antibiotic.The bacterial challenge test was carried out on SPF mice with the isolated strains.It was found that the isolated bacteria had strong pathogenicity to mice, resulting in congestion and swelling of the lungs and kidneys, intestinal lesions and yellow thin stools.【Conclusion】 The results of this study showed that Staphylococcus chromogenes isolated from yaks in Lhasa area had a certain pathogenicity and strong drug resistance, which should be paid more attention.

Key words: yak; Staphylococcus chromogenes; isolation and identification

CLC Number:

S852.61

ZOU Minghao, MA Hongcai, WANG Bingyi, FAN Shijie, ZENG Jiangyong, WU Qingxia. Isolation, Identification and Drug Resistance Analysis of Staphylococcus chromogenes in Yak[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 3017-3025.

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Isolation, Identification and Drug Resistance Analysis of Staphylococcus chromogenes in Yak

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