催乳素对内蒙古绒山羊毛囊体外培养影响的研究

doi:10.16431/j.cnki.1671-7236.2022.06.007

Abstract

Abstract: 【Objective】 The aim of this experiment was to study the effects of prolactin (PRL) on the growth and morphological changes of primary and secondary hair follicles in Inner Mongolia cashmere goats in vitro.【Method】 The primary and secondary hair follicles of Inner Mongolia cashmere goats were isolated by mechanical method combined with cutting method.The primary hair follicle culture medium was supplemented with 0, 5, 10, 50 and 100 ng/mL prolactin, respectively, with 24 roots in each group for 5 days in vitro culture.The morphology of the hair follicles was observed and photographed under a microscope every day.The growth length, growth rate and survival rate were calculated to screen out the optimal concentration of prolactin.Then, the primary and secondary hair follicles were divided into primary hair follicle control group (PF-K), primary hair follicle experimental group (PF-PRL), secondary hair follicle control group (SF-K) and secondary hair follicle experimental group (SF-PRL), with 24 hairs in each group.The control group was cultured with the basal medium, and the experimental group medium was supplemented with the optimal concentration of prolactin.The hair follicles were cultured for 5 days, the morphology of hair follicles were observed and took photos every day, and the growth length of each group was measured at the same time.【Result】 The average daily growth length of hair follicle in 10 ng/mL prolactin group was extremely significantly higher than that in other concentration groups, the final growth length and survival rate of hair follicles in 10 ng/mL prolactin group were the highest (P<0.01).Therefore, 10 ng/mL prolactin was selected for subsequent experiments.The stem and root sheath of primary/secondary hair follicles in experimental and control groups were elongated simultaneously, and curved to different degrees with the increase of culture time.The total length of hair follicles PF-PRL and SF-PRL groups at 2-5 days was significantly higher than that in PF-K and SF-K groups, respectively (P<0.01).The total length of hair follicles in PF-K group increased significantly from day 1 to 5, except that there was no significant difference between day 1 and 0 (P<0.05), the total length of hair follicles in PF-PRL group was increased significantly from 0 to 5 days (P<0.05).The total length of hair follicles in SF-K group on the 5th day was significantly higher than that in 0-4 days (P<0.05).The total length of hair follicles in SF-PRL group was significantly higher than 0-3 days on day 4 and 5 (P<0.05), and the total length of hair follicles on day 3 was significantly higher than 0-2 days (P<0.05).The average daily growth length of hair follicles in PF-PRL and SF-PRL groups was significantly higher than that in PF-K and SF-K groups (P<0.01).【Conclusion】 10 ng/mL prolactin was the optimal concentration to promote hair follicle growth in vitro.10 ng/mL prolactin could promote the growth of primary and secondary hair follicles of Inner Mongolia cashmere goats in vitro.

Key words: prolactin; Inner Mongolia cashmere goat; hair follicle; in vitro culture

CLC Number:

S857.4

BAO Yuexian, ZHAO Feifei, GONG Wendian, LU Zeyu, MU Qing, LIU Junyang, TE Rigele, ZHAO Yanhong. Effects of Prolactin on Hair Follicle Culture of Inner Mongolia Cashmere Goats in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2072-2078.

References

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Effects of Prolactin on Hair Follicle Culture of Inner Mongolia Cashmere Goats in vitro

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