China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (8): 3019-3029.doi: 10.16431/j.cnki.1671-7236.2021.08.035

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Genetic Variation Analysis of Major Virulence Genes of Pseudorabies Virus Epidemic Strains in Southern Henan Province

QU Zhehui1, ZHANG Xiwen1, ZHAO Yu1, ZHENG Quanfang1, LIAN Huixiang1, ZHAO Yunhuan1,2,3, GUO Xiaoqiu1   

  1. 1. College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang 464000, China;
    2. Engineering Research Center for Health Production of Ecological Livestock and Poultry of Henan Dabieshan District, Xinyang 464000, China;
    3. Xinyang Key Laboratory of Animal Husbandry and Environmental Control, Xinyang 464000, China
  • Received:2021-01-25 Online:2021-08-20 Published:2021-08-19

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Abstract: In order to investigate the molecular genetic characteristics of Pseudorabies virus (PRV) in Southern Henan province, the main PRV virulence genes gB, gE, gC, gD and TK from the tissues of PRV-infected pigs were amplified by PCR and sequenced.The similarity, phylogenetic tree and amino acid variation analysis of major virulence genes between epidemic strains and published reference sequences were performed by MegAlign software.Sequencing results showed that the PRV gB, gE, gC, gD and TK genes from 5 PRV-infected pig tissues were successfully amplified.Amino acid similarity analysis and phylogenetic tree results showed that the 5 PRV epidemic strains in Southern Henan province were in G1 group with the domestic epidemic strains, the amino acid similarity of gB, gE, gC and gD were 98.5%-100%, 97.2%-100%, 97.5%-100% and 98.3%-100%, respectively, relatively distantly related to G2 group (the Bartha strain, Becker strain, and NiA3 strain in Europe and America), the amino acid similarity of gB, gE, gC and gD were 96.4%-97.4%, 94.6%-95.7%, 92.7%-94.0% and 96.3%-99.0%, respectively.Compared with the Bartha-K61 strain (or Becker strain, NiA3 strain, etc.), there were amino acids deletion, insertion and mutation in the 5 epidemic strains, such as ‘PGL’ deletion at position 75-77 and ‘G’ insertion at position 94 in gB amino acids, the ‘D’ insertions at positions 48 and 496 in gE amino acid sequence, the ‘VSGTTGA’ insertions at positions 57-63 and the ‘SPEAG’ mutation to ‘ASTPA’ at positions 65-69 in the gC amino acid, the ‘RP’ or ‘RPRP’ insertion at positions 278-281 in gD amino acid.In addition, there were single amino acid mutations in gB, gE, gC, gD and TK amino acid, respectively.Therefore, the epidemic strains of PRV in Southern Henan province showed the molecular genetic characteristics of the variant strains.The above results confirmed that the 5 PRV epidemic strains were variant strains, which were relatively distantly related to the vaccine strain Bartha-K61.

Key words: Pseudorabies virus (PRV); similarity analysis; genetic variation; virulence genes; variant strains

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