睾丸支持细胞的功能、分离纯化及鉴定研究进展

doi:10.16431/j.cnki.1671-7236.2021.08.027

Abstract

Abstract: Sertoli cells play a decisive role in maintaining the microenvironment in the process of spermatogenesis.They can promote the process of spermatogenesis through secretion function, blood testis barrier function formed by intercellular connection and phagocytosis, abnormal development of them will lead to different degrees of male reproductive defects.Based on the role of Sertoli cells in the reproductive process of male animals, highly purified Sertoli cells cultured in vitro can be an important cell model to study the two core functions of testis spermatogenesis and sex hormone secretion.In addition, purified Sertoli cells cultured in vitro can also be used as a cell model in toxicology and other newly developing hot fields to provide convenience for assessing and researching on the effects of environmental factors on male reproduction.In this article, the current research progress on the function of Sertoli cell and its commonly methods of in vitro isolation, purification, culture and characterization were systematically summarized to provide references for the use of animal Sertoli cells in the studies of male reproduction.

Key words: Sertoli cells; function; isolation and purification; in vitro culture; identification

CLC Number:

S827

YI Minna, Ulaangerel Tseweendolmaa, Toli Davshilt, WANG Xisheng, SHEN Yingchao, DU Ming, REN Hong, MANG Lai, BOU Gerelchimeg. Research Progress on Function,Isolation,Purification and Identification of Sertoli Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2947-2956.

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Research Progress on Function,Isolation,Purification and Identification of Sertoli Cells

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