吉林地区1株小鹅瘟病毒NS1和VP1基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2021.04.002

Abstract

Abstract: In order to understand the genetic characteristics and the correlation of Gosling plague virus (GPV) from Jilin district with isolated strains from Heilongjiang and other provinces of China and abroad,the intestinal tissue of dead goslings from Jilin district in 2018 was detected by PCR.At the same time,the non-structural protein NS1 gene and structural protein VP1 gene of GPV were cloned and sequenced.Sequence analysis were conducted with the corresponding sequence of 16 GPV reference strains of China and abroad.The results showed that the mixed infection of GPV and EDSV was confirmed in dead gosling.The NS1 gene of GPV from Jilin was 1 884 bp in length,encoded 627 amino acids,and the nucleotide homology with the reference strains were 93.8% to 99.8%,and the amino acid homology were 97.1% to 99.7%.The VP1 gene of GPV was 2 199 bp in length,encoded 732 amino acids,and the nucleotide homology with the reference strains were 93.4% to 99.9%,and the amino acid homology were 96.4% to 99.9%.The phylogenetic tree analysis of NS1 and VP1 genes showed that GPV from Jilin and Harbin isolated 98E strain belonged to the same evolutionary branch,and the relationship was the closest,but far away from abroad,Taiwan and Anhui isolated strains.The NS1 and VP1 amino acid sequence of GPV from Jinlin were analyzed with GPV origined different kinds of waterfowls,the results showed that GPV from Jilin had a closer relationship and the higher homology with goose-origin parvovirus.This study provided basic date for clarifying the spatial epidemic regularity of GPV in Northeast China,and provided a reference basis for the diagnosis and treatment of GPV in Northeast China.

Key words: Gosling plague virus (GPV); gene; clone; sequence analysis; mixed infection

CLC Number:

S852.65⁺9.2

ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.

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Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District

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