奶牛活体采卵-体外受精效率的影响因素研究

doi:10.16431/j.cnki.1671-7236.2021.02.019

Abstract

Abstract: The aim of this study was to optimize the ovum pick-up (OPU) and in vitro fertilization system,and further improve the in vitro embryo production efficiency.The optimal in vitro culture medium was screened out by using fresh ovaries collected from slaughterhouses for in vitro fertilization.The effects of different bull semen and donor cows on the outcome of in vitro fertilization of oocytes collected by OPU were analyzed.The results showed that compared with CR1aa medium,mCR1aa medium had no significant effect on cleavage rate,but the blastocyst formation rate was significantly increased (28.1% vs 20.6%,P<0.05).Three Holstein bulls semen were randomly selected for in vitro fertilization,the proportion of cleaved embryos was not affected,but the blastocyst development rate of in vitro-produced embryos originating from No.1 bull semen (38.7%) was significantly higher than that of No.2 (23.8%) and No.3 bull semen (22.9%) (P<0.05).There was no significant difference in the average number of recovered oocytes obtained from three randomly selected donor cows (H1,H2 and H3),but the developmental ability of embryos after in vitro fertilization was significantly different.The cleavage rates blastocyst rates of H1 and H2 donors were significantly higher than that of H3 donors (P<0.05),and the blastocyst rate of H1 donor was significantly higher than that of H2 donor (P<0.05).In summary,mCR1aa medium significantly increased the blastocyst development rate,and could be used for in vitro embryo production,the individual variability in bull semen and donor cows could directly affect the production efficiency of in vitro embryo production.The results provided a reference for the optimization of OPU-in vitro fertilization technology.

Key words: dairy cow; ovum pick-up; in vitro fertilization; in vitro embryo; blastocyst

CLC Number:

S823.9⁺1

WANG Tengfei, ZHANG Yan, WANG Yanping, ZHAO Shanjiang, ZHU Huabin, WANG Lingling, MA Zhu, DAI Yunping, PANG Yunwei. Study on the Influencing Factors of in vitro Fertilization Efficiency in Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 574-580.

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Study on the Influencing Factors of in vitro Fertilization Efficiency in Dairy Cows

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