乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究

doi:10.16431/j.cnki.1671-7236.2021.02.009

Abstract

Abstract: The effects of Dark Plum powder extraction on the contraction of small intestine in vitro were investigated and the possible mechanism was explored.The water extraction was prepared by decocting the following Chinese herbs including 30 g of Dark Plum,50 g of Calyx Kaki,12 g of Medicine Terminalia Fruit,12 g of Coptidis Rhizoma,and 12 g of Radix Curcumae.The technique of isothermal perfusion of small intestine in vitro was used.RM6240 multi-channel biological signal acquisition and processing system was used for information collection and processing.The effects of Dark Plum powder extraction on the spontaneous contraction of isolated gastrointestinal smooth muscles were observed.The effects of 2.18 g/L of Dark Plum powder extraction on the contraction induced by acetylcholine (Ach) were measured.The effects of 2.18 g/L of Dark Plum powder extraction on the Ach-induced both intracellular and extracellular calcium-depended contractions were studied.The amplitude and frequency of spontaneous contraction of isolated gastrointestinal smooth muscles were significantly reduced by Dark Plum powder extraction at the concentration of more than 1.09 g/L (P<0.05).Ach observably induced the amplitude of smooth muscle contractions (P<0.01).Dark Plum powder extraction at the concentration of 2.18 g/L markedly inhibited the amplitude and frequency of spasmodic contractions (P<0.01).In the Ca²⁺ free tyrodes solution,Dark Plum powder extraction evidently inhibited the amplitude and frequency of the Ach-induced both intracellular and extracellular calcium-depended contractions (P<0.01).Dark Plum powder extraction inhibited the spontaneous contraction and spasmodic contraction caused by Ach in a dose-dependent manner,isolated gastric smooth muscle.The mechanism was speculated to be related to inhibiting the extracellular Ca²⁺ inflowing via cell membrane and intracellular Ca²⁺ releasing.

Key words: Dark Plum powder; gastrointestinal smooth muscle; calcium channel

CLC Number:

S829.1

SHANG Shufeng, WANG Qi, GUO Sufen, CHEN Zhiyuan, WANG Yangke. Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 477-483.

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Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism

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