四甲基吡嗪对獭兔单增李斯特菌感染和天然免疫的影响

doi:10.16431/j.cnki.1671-7236.2020.05.028

Abstract

Abstract: The study aimed to investigate the effect of tetramethylpyrazine(TMP) supplementation on Listeria monocytogenes loads,listeriolysin O (LLO),proinflammatory factors (TNF-α,IL-1β and IFN-γ),immune organ index and natural immunoglobulins of Rex rabbits infected with Listeria monocytogenes.A total of 180 Rex rabbits were randomly assigned to 5 groups with 6 replicates of 6 rabbits.The control group was fed with the basal diet,and the other four groups were fed with the addition of TMP at 0,50,100 and 150 mg/kg of diet,respectively.Each Rex rabbit in the four treatments was fed with 1 mL (10⁷ CFU) of Listeria monocytogenes,and the control group was fed with the culture medium of aseptic strainat first day of feeding trial.The trial lasted for 14 days.The results showed that TMP addition decreased the loads of Listeria monocytogenes in the spleen,liver and lymph nodes (P<0.05).The pathogen loads in caecal content,liver,spleen and lymph nodes linearly decreased (P<0.05).Also,TMP decreased serum LLO levels and proinflammatory factors TNF-α and IL-1β (P<0.05).Linearly decreased for LLO and linearly and quadratically decreased for TNF-α and IL-1β (P<0.05) at 14 d.The indexes of spleen and liver and the serum levels of IgA and IgG inhanced with the increasing doses of TMP,but the spleen index on 7 d post administration linearly decreased (P<0.05).In conclusion,supplemental TMP in the rabbit diet could attenuate Listeria monocytogenes infection by reducing the pathogen loads and virulence,increasing immune organ indexes and natural immunoglobulins.

Key words: TMP; Listeria monocytogenes; bacterial load; innate immunoglobulins; Rex rabbit

CLC Number:

S829.1

ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Tetramethylpyrazine on Listeria monocytogenes Infection and Innate Immunity of Rex Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1531-1538.

References

[1] SWMINATHAN B,GERNER-SMIDT P.The epidemiology of human listeriosis[J].Microbes & Infection,2007,9(10):1236-1243.
[2] CHENG C,WANG H,MA T,et al.Flagellar basal body structural proteins FlhB,FliM,and FliY are required for flagellar-associated protein expression inListeria monocytogenes[J].Front Microbiol,2018,9:208.
[3] 崔焕忠,乔立桥,王义冲.单核细胞增生性李斯特菌的主要毒力因子及其致病机理[J].中国畜牧兽医,2010,37(1):128-133. CUI H Z,QIAO L Q,WANG Y C.Main virulence factors and pathopoiesis mechanism of Listeria monocytogenes[J].China Animal Husbandry & Veterinary Medicine,2010,37(1):128-133.(in Chinese)
[4] PAPIĆ B,GOLOB M,KUŠARD,et al.Source tracking on a dairy farm reveals a high occurrence of subclinical mastitis due to hypervirulent Listeria monocytogenes clonal complexes[J].Journal of Applied Microbiology,2019,127:1349-1361.
[5] 李善高,曹海军,刘军,等.四甲基吡嗪对大鼠肝星状细胞肌细胞增强因子2A及α-平滑肌肌动蛋白表达的影响[J].中国中西医结合消化杂志,2012,20(8):337-341. LI S G,CAO H J,LIU J,et al.Effectortetramethylpyrazine on the expression of myocyte enhancer factor 2A and α-smooth muscle actin in process of hepatic stellate cell activation[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2012,20(8):337-341.(in Chinese)
[6] 李善高,王天才,梁扩寰,等.四甲基吡嗪对实验性大鼠肝纤维化防治作用的研究[J].中华消化杂志,1998,18(3):153-155. LI S G,WANG T C,LIANG K H,et al.Effects of prevention and treatment of tetramethylpyrazine on experimental liver fibrosis in rats[J].Chinese Journal of Digestion,1998,18(3):153-155.(in Chinese)
[7] LI H,XUE Y,WEI S,et al.Protective effects of tetramethylpyrazine on cerebrovascular regulations in rats with chronic alcoholic encephalopathy[J].Biomedical and Environmental Sciences,2015,28(9):691-695.
[8] LIU N,LIN L,WANG J Q,et al.Tetramethylpyrazine supplementation reduced Salmonella typhimurium load and inflammatory response in broilers[J].Poultry Science,2019,98:3158-3164.
[9] LUKIC J,JANCIC I,MIRKOVIC N,et al.Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes[J].Beneficial Microbes,2017,8:809-822.
[10] GELBĆÍOVÁ T,FLORIANOVlÁ M,TOMÁŠTÍKOVÁ Z,et al.Prediction of persistence of Listeria monocytogenes ST451 in arabbitmeat processing plant in the Czech Republic[J].Journal of Food Protection,2019,82(8):1350-1356.
[11] DE CESARE A,PARISI A,MIONIR R,et al.Listeria monocytogenescirculating in rabbit meat products and slaughterhouses in Italy:Prevalence data and comparison among typing results[J].Foodborne Pathogens and Disease,2017,14(3):167-176.
[12] KONÉ A P,ZEA J M V,GAGNÉ D,et al.Application of Carnobacteriummaltaromaticum as a feed additive for weaned rabbits to improve meat microbial quality and safety[J].Meat Science,2018,135:174-188.
[13] 林茹.四甲基吡嗪干预实验性自身免疫性葡萄膜炎的作用及其机制的研究[D].重庆:重庆医科大学,2017. LIN R.TMP attenuatesintraocular inflammationin experimental autoimmune uveitis through modulating Stat3 and Stat4 pathways[D].Chongqing:Chongqing Medical University,2017.
[14] 王万铁,林丽娜.川芎嗪对心脏换瓣患者血小板聚集功能的影响[J].中国中西医结合杂志,2000,20(1):25. WANG W T,LI L N.Effect of Ligustrazine on platelet aggregation in patients with heart valve replacement[J].Chinese Journal of Integrative Medicine,2000,20(1):25.(in Chinese)
[15] 吴旭,喻惠芳.抵克力得与川芎嗪对缺血性脑卒中血小板聚集性的影响[J].湖北中医药大学学报,2001,3(3):29. WU X,YU H F.Effects of Ticlopidine and Ligustrazine on platelet agglutination in patients with ischemic cerebral infarction[J].Jounal of Hubei College of Trditional Chinese Medicine,2001,3(3):29.(in Chinese)
[16] 刘晓丽,霍展样,张金莲.盐酸川芎嗪体外抗氧化作用的研究[J].中国现代医药杂志,2003,5(2):37-39. LIU X L,HUO Z X,ZHANG J L.Antioxidative activity of ligustrazine in vitro[J].Modern Medicine Journal of China,2003,5(2):37-39.(in Chinese)
[17] HAMON M A,RIBET D,STAVRU F,et al.Listeriolysin O:The swiss army knife oflisteria[J].Trends in Microbiology,2012,20(8):360-368.
[18] SEVEAU S.Multifaceted activity of listeriolysin O,the cholesterol-dependentcytolysin of Listeria monocytogenes[J].Subcell Biochem,2014,80:161-195.
[19] KORTEBI M,MILOHANIC E,MITCHELL G,et al.Listeria monocytogenes switches from dissemination to persistence by adopting a vacuolar lifestyle in epithelialcells[J].PLoS Pathog,2017,13(11):e1006734.
[20] OSBORNE S E,BRUMELL J H.Listeriolysin O:from bazooka to Swiss army knife[J].Philosophical Transactions of the Royal Society B:Biological Sciences,2017,8:372.
[21] NGUYEN B N,PETERSON B N,PORTNOY D A.Listeriolysin O:A phagosome-specific cytolysinrevisited[J].Cellular Microbiology,2019,21:e12988.
[22] MEIXENBERGER K,PACHE F,EITEL J,et al.Listeria monocytogenesinfected human peripheral blood mononuclear cells produce IL-1,depending on listeriolysin O and NLRP3[J].The Journal of Immunology,2009,184:922-930.
[23] HORAI R,CASPI R R.Cytokines in autoimmune uveitis[J].Journal of Interferon & Cytokine Research,2011,31(10):733-744.
[24] IYER S S,CHENG G.Role of interleukin 10 transcriptional regulation ininflammation and autoimmune disease[J].Critical Reviews in Immunology,2012,32(1):23-63.
[25] COPE A,LE F G,CARDONG J,et al.The Th1 life cycle:Molecular control of IFN-γ to IL-10 switching[J].Trends in Immunology,2011,32(6):278-286.
[26] RIZZO L V,XU H,CHAN C C,et al.IL-10 has a protective role in experimental autoimmuneuveoretinitis[J].International Immunology,1998,10(6):807-814.
[27] PUERTOLLANO E,PUERTOLLANO M A,CRUZ-CHAMORRO L,et al.Orally administered Lactobacillus plantarum reduces proinflammatory interleukin secretion in sera from Listeria monocytogenes infected mice[J].British Journal of Nutrition,2008,99:819-825.
[28] CHOI H J,SHIN M S,LEE S M,et al.Immunomodulatory properties of Enterococcus faecium JWS 833 isolated from duck intestinal tract and suppression of Listeria monocytogenes infection[J].Microbiology and Immunology,2012,56:613-620.
[29] POPOVIĆ N,DJOKIĆ J,BRDARIĆ E,et al.The influence of heat-killed Enterococcus faecium BGPAS1-3 on the tight junction protein expression and immune function in differentiated caco-2 cells infected with Listeria monocytogenes ATCC 19111[J].Frontiers in Microbiology,2019,10:412.
[30] OKAMOTO M,NAKANE A,MINAGAWA T.Host resistance to an intragastric infection with Listeriamonocytogenesin mice depends on cellular immunity and intestinal bacterial flora[J].Infection and Immunity,1994,62:3080-3085.
[31] CORR S C,HILL C,GAHAN C G M,et al.Chapter 1 understanding the mechanisms by which probiotics inhibit gastrointestinal pathogens[J].InAdvances in Food and Nutrition Research,2009,6:1-15.
[32] LEBEER S,BRON P A,MARCO M L,et al.Identification of probiotic effector molecules:Present state and future perspectives[J].Current Opinion in Biotechnology,2018,49:217-223.

Effect of Tetramethylpyrazine on Listeria monocytogenes Infection and Innate Immunity of Rex Rabbits

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZENG Dongdong, LIU Caixia, KOU Lijun, QIN He, JIN Ruijie, WANG Jing, REN Jingjing, MA Xun, JIANG Jianjun. Construction and Biological Characteristics of Lm4b_02324 Gene Deletion Strains of Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1981-1990.
[2]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	GUO Qian, ZHANG Yu, FANG Xiaowei, AN Ni, YUAN Mei, FANG Chun. Study on Glycosyl-flippase gtcA Gene Deletion Reduces the Pathogenicity of Listeria monocytogenes Serotype 1/2a [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2298-2306.
[5]	WEI Xiaojun, LI Qiaoqiao, ZENG Dongdong, JIANG Jianjun. Construction of Listeria monocytogenes inlK Gene Deletion Strain and Analysis of Its Biofilm and Disinfectant Resistance [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1870-1878.
[6]	CHEN Lingbo, ZHANG Yu, FANG Xiaowei, JI Junzhi, FANG Chun, YANG Yuying. Effects of the Two-component System LisRK on Acid Resistance and Invasiveness of Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 416-423.
[7]	ZHENG Jiangping, LIU Ning, BAI Xuerui, SHANG Yueli. Effects and Mechanism of Lactobacillus plantarum Culture Against Listeria monocytogenes Infection in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2666-2676.
[8]	ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Yeast Polysaccharide on Growth Performance,Bacterial Load and Endogenous Antimicrobial Peptides in Rabbits Infected with Campylobacter jejuni Expression [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1099-1108.
[9]	LIU Ning, FU Hualin, LIU Hanzhong, LI Rui, ZHANG Kai, YU Zhiju, JIAN Wensu, WANG Ping, FU Xiangchao, DU Dan, XU Changwen. Effects of Different Chinese Herbal Compound on Growth Performance and Serum Biochemical Indexes of Weaned Rex Rabbits [J]. , 2018, 45(2): 422-428.
[10]	WANG Liang, FAN Hong-jie. Establishment of the Rex Rabbit Fluorosis Model and Detection of the Fluoride Content in Organs [J]. , 2017, 44(7): 2160-2164.
[11]	YE Xiang-yang, XIE Bao-cai, REN Zhan-jun, BAI Jing. Effect of Chinese Herbal Medicine Additives on Milk Production of Rex Rabbit [J]. , 2017, 44(12): 3525-3529.
[12]	ZHAO Yue-ping, YANG Cui-jun, SUN Quan-wen, WU Shu-qin, WU Zhan-fu, HAO Rong-chao, LU Jing, MA Meng-pan, REN Ling-ling. Comparison of Production Performance of Different Mating Combination Between Castor Rex Rabbit and Plicae Rex Rabbit [J]. , 2017, 44(12): 3578-3584.
[13]	YANG Yang, LI Wen-hui, ZHANG Nian-zhang, LI Ting-ting, LI Li, YAN Hong-bin, JIA Wan-zhong, FU Bao-quan. Cloning and Prokaryotic Expression of Protein Kinase A Catalytic Subunit Gene from Taenia multiceps [J]. , 2017, 44(10): 2858-2864.
[14]	LI Dan-dan, XU Yi-gang, LI Meng-yuan, WANG Yu, QIU Suo-ping, GAO Hui-jiang, GAO Shen-yang. Development of A Dual Real-time PCR for the Rapid Detection of Listeria monocytogenes [J]. , 2016, 43(6): 1453-1457.
[15]	CHEN Mei-zhen, ZHANG Jia, WANG Shou-kun, LIN Xuan, CHEN Ren-feng, HU Ling-ying, CHEN Lin-liu, XIE Zhao-wen, WEN Fu-li. Epidemiological Investigation and Species Identification of Eimeria of a Rex Rabbit Farm in Fujian Province [J]. , 2015, 42(8): 2204-2209.